국내 육성 품종 ‘탐미유채’의 소포자 배양 시 배양조건이 배발생에 미치는 영향

• Published in: Korean journal of crop science Vol. 57; no. 4; pp. 317 - 323
• Main Authors: 김광수, Kwang-soo Kim, 이영화, Yong-hwa Lee, 조현준, Hyeon-jun Cho, 장영석, Young-seok Jang, 박광근, Kwang-geun Park
• Format: Journal Article
• Language: Korean
• Published: 한국작물학회 31.12.2012
• Subjects: Brassica napus; culture density; embryogenesis; heat treatment; microspore culture; regeneration; 농학; Brassica napus; embryogenesis; heat treatment; regeneration; microspore culture; culture density
• ISSN: 0252-9777; 2287-8432
• DOI: 10.7740/kjcs.2012.57.4.317

For the establishment of an efficient embryogenesis from microspore culture in Brassica napus L. domestic cultivar ‘Tammiyuchae’, four different factors affecting microspore embryogenesis and plantlet regeneration were investigated. The highest embryogenesis rate was achieved when microspores at late uninucleate to early binucleate stage were isolated from flower buds with a length of 3.0～3.5 mm. On average, 388 embryos generated from 1 ml of microspores media. The highest number of embryos was obtained when microspores were subjected to 32.5℃ for 2 days. Embryogenesis of ‘Tammiyuchae’ was increased with increasing microspore culture density up to about 5 × 10 4 ea/mL. Gradually higher culture density repressed embryogenesis of microspores. Regeneration rate of shoots from microspore-derived embryos was observed in MS solid medium supplemented with 0.5 mg · L -1 NAA and 1.0 mg · L -1 BA, and grew well in MS solid medium without plant growth regulators.