COMPARISON OF THE IN VITRO QUALITIES OF PLATELET CONCENTRATES SUSPENDED IN T-PAS+AND BICANATE COLLECTED WITH TRIMA ACCEL

• Published in: Japanese Journal of Transfusion and Cell Therapy Vol. 63; no. 6; pp. 780 - 787
• Main Authors: Onodera, Hidekazu, Kaneko, Yuji, Koike, Toshiyasu, Miyajima, Haruko, Moriyama, Rie, Chatani, Makoto, Nishitani, Yumiko, Hirayama, Jyunichi, Shibata, Reiko, Shiba, Masayuki, Nagai, Tadashi, Satake, Masahiro, Tadokoro, Kenji
• Format: Journal Article
• Language: Japanese
• Published: The Japan Society of Transfusion Medicine and Cell Therapy 25.12.2017
• Subjects: Bicanate; PAS; PAS-PC; T-PAS
• ISSN: 1881-3011; 1883-0625
• DOI: 10.3925/jjtc.63.780

Platelet concentrate suspended in Platelet Additive Solution (PAS) is a collection in which part of the plasma is replaced with PAS, and the residual plasma concentration is approximately 20% to 35% of the total volume. Platelet concentrate suspended in PAS (PAS-PC) is reportedly useful for decreasing the incidence of allergic side effects caused by transfusion. In this study, we investigated whether it is feasible to prepare PAS-PC using the blood component collection device Trima Accel. We collected ten platelet components, targeting a 200 ml final volume, 2.5×1011 total number of platelets per bag, and 35% residual plasma concentration. We obtained an average (range), volume of 197 ml (194-201 ml), a total platelet count of 2.69×1011/bag (2.49-3.00×1011/bag), and a residual plasma concentration of 35.0% (33.0-36.2%). Collection of PAS-PC according to the set goals was thus achievable. We examined the quality of PAS-PC collected from the same donor with the addition of either T-PAS+or Bicanate. The quality of the preparation was well maintained up until day 7 with T-PAS+. In contrast, the pH was more than 7.7 with Bicanate, which was higher than with T-PAS+, and glucose depletion was observed on day 7, and the number of platelets with spheroidal morphology was also higher.