EVALUATION OF VARIOUS APHERESIS MACHINES IN PLATELETPHERESIS WITH SPECIAL REFERENCE TO LEUKOCYTE CONTAMINATION IN PLATELET CONCENTRATE MEASURED BY FLOW CYTOMETRY

• Published in: Jinko Zoki Vol. 23; no. 2; pp. 518 - 522
• Main Authors: OMOKAWA, S., NOTOYA, T., MIURA, A.b.
• Format: Journal Article
• Language: Japanese
• Published: JAPANESE SOCIETY FOR ARTIFICIAL ORGANS 15.04.1994
• Subjects: flow cytometric analysis; platelet concentrate; plateletpheresis; white blood cell contamination
• ISSN: 0300-0818; 1883-6097
• DOI: 10.11392/jsao1972.23.518

The objective of this study is to compare the efficiency of two plateletpheresis machines in terms of white blood cell (WBC) contamination. Plateletpheresis was performed in normal donors using COBS Spectra and HAEMONETICS V-50. Biochemical and hematological assays, serum hemoglobin and C3a, C5a were measured before and after the procedure. WBC contamination in the platelet concentrate product was studied using a Coulter counter (conventional) and a Cytron Absolute (flow cytometric analysis; FCM). Procedure time was shorter in Spectra (73.5min) than in V-50 (105.7 min), but platelet yield was comparable. C3a and C5a were not increased after procedure both in Spectra and V-50. WBC contamination assayed by Coulter counter was significantly higher in V-50 (683.3/μl) than in Spectra (not detected). FCM could detect extremely low concentration of WBC (<50/μl) compared with the conventional assay. WBC in the platelet concentrate product was 1.04×105/product in Spectra measured by FCM. The data suggested that Spectra was more effective in collecting platelet with less WBC contamination and that FCM was useful method to evaluate WBC contamination in the platelet concentrate by apheresis.