Evaluation of autoimmune sensorineural hearing loss using a lymphocyte activation assay

Peripheral lymphocytes were isolated from patients with progressive sensorineural hearing loss of unknown origin. The peripheral lymphocytes were cocultured for 120 hours with human inner ear tissue which had been obtained earlier by petrosectomy. 3H-Thymidine was added to the culture for the last 4...

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Bibliographic Details
Published injibi to rinsho Vol. 37; no. 3; pp. 543 - 549
Main Authors KOJA, Shizuo, CHINEN, Nobuo, URA, Masaharu, BERGER, Peter
Format Journal Article
LanguageJapanese
Published JIBI TO RINSHO KAI 20.05.1991
Subjects
autoimmune sensorineural hearing loss
lymphocyte activation test
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Summary:Peripheral lymphocytes were isolated from patients with progressive sensorineural hearing loss of unknown origin. The peripheral lymphocytes were cocultured for 120 hours with human inner ear tissue which had been obtained earlier by petrosectomy. 3H-Thymidine was added to the culture for the last 4 hours, and the radioactivity of activated lymphocytes was counted. Cultures which exceed twice the counts of control cultures were rated as positives. The results were as follows: 1. In 50 patients with progressive sensorineural hearing loss of unknown origin, 15 patients were rated positive. None of the control cultures were detected as positive. 2. It is suggested that cellular immunity is one of the possible causes of sensorineural hearing loss. 3. The lymphocyte activation assay is considered to be a useful method for evaluating sensorineural hearing loss caused by cellular immunity.
ISSN:0447-7227
2185-1034
DOI:10.11334/jibi1954.37.3_543