Expression of a Chemically Synthesized Human α1Interferon Gene

Cells of Escherichia coli containing a chemically synthesized human α1interferon (IFN-α1) gene, under control of the lac promoter, make a product with biological properties indistinguishable from those of the natural IFN-α1[antiviral activity, acid stability, species crossreactivity, inactivation by...

Full description

Saved in:
Bibliographic Details
Published inProceedings of the National Academy of Sciences - PNAS Vol. 79; no. 14; pp. 4256 - 4259
Main Authors De Maeyer, Edward, Skup, Danial, Kolli S. N. Prasad, De Maeyer-Guignard, Jaqueline, Williams, Bryan, Meacock, Peter, Sharpe, Geoff, Pioli, Dave, Hennam, John, Schuch, Wolfgang, Atherton, Keith
Format Journal Article
LanguageEnglish
Published National Academy of Sciences of the United States of America 15.07.1982
Subjects
Antivirals
Cell lines
Complementary DNA
Genes
Genetic vectors
Humans
Interferons
Plasmids
Synthetic genes
Ungulates
Online AccessGet full text

Cover

More Information
Summary:Cells of Escherichia coli containing a chemically synthesized human α1interferon (IFN-α1) gene, under control of the lac promoter, make a product with biological properties indistinguishable from those of the natural IFN-α1[antiviral activity, acid stability, species crossreactivity, inactivation by antisera directed against leukocyte or Namalwa cell interferon, and stimulation of (2′-5′)oligoadenylate synthetase activity]. Similar levels of IFN synthesis were obtained when the expression unit (lac promoter plus synthetic IFN-α1gene) was transplanted into the obligate methylotroph Methylophilus methylotrophus.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.79.14.4256