Validations of Adhesive Films for Two-dimensional Elemental Analyses of Bone Tissue

• Published in: Advances in X-Ray Chemical Analysis, Japan Vol. 50; pp. 313 - 319
• Main Authors: ISHIHARA, Hiroshi, NUMAKO, Chiya, OIKAWA, Masakazu, YOSHIDA, Takanori, HOMMA-TAKEDA, Shino, UEHARA, Akihiro
• Format: Journal Article
• Language: Japanese
• Published: The Discussion Group of X-Ray Analysis, The Japan Society for Analytical Chemistry 31.03.2019; 公益社団法人 日本分析化学会 X線分析研究懇談会
• Subjects: 2次元元素分析; Adhesive films; Bone; PIXE; Two-dimensional elemental analyses; 粘着性フィルム; 骨
• ISSN: 0911-7806; 2758-3651
• DOI: 10.57415/xshinpo.50.0_313

Three adhesive films, Kawamoto film used for histopathological sections of hard tissues, Kapton film, and polypropylene film were examined for preparation of thin section specimens and compared by microscopic observation and PIXE analysis of contained elements. Cryo-sections could be obtained for these films by appressing each films strongly to the surfaces of the frozen samples. By microscopic observation, cryo-sections on Kapton film suited for two-dimensional elemental analyses, while fine cracks were slightly occurred in the soft tissues than those on Kawamoto film. In contrast, a small part of the soft tissues on polypropylene film was missed. Kawamoto and polypropylene films contained biological essential elements, such as Ca, Fe and Zn slightly. These results suggested that the adhesive Kapton film was most suitable for two-dimensional elemental analyses.