The cbfs triple mutants reveal the essential functions of CBFs in cold acclimation and allow the definition of CBF regulons in Arabidopsis

• Published in: The New phytologist Vol. 212; no. 2; pp. 345 - 353
• Main Authors: Jia, Yuxin, Ding, Yanglin, Shi, Yiting, Zhang, Xiaoyan, Gong, Zhizhong, Yang, Shuhua
• Format: Journal Article
• Language: English
• Published: England New Phytologist Trust 01.10.2016; Wiley Subscription Services, Inc
• Subjects: Acclimation; Acclimatization; Acclimatization - genetics; Arabidopsis; Arabidopsis - genetics; Arabidopsis - physiology; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Base Sequence; CBF regulons; Cellular stress response; Chilling; chilling response; Cold; Cold acclimation; Cold Temperature; Cold tolerance; Cooling; CRISPR; C‐repeat binding factor (CBF) transcription factors; Deoxyribonucleic acid; DNA; Freezing; Gene editing; Gene Expression Regulation, Plant; Genes; Genes, Plant; Insertion; Mutants; Mutation - genetics; Rapid reports; Regulon - genetics; Sequence Analysis, RNA; Stress, Physiological - genetics; Transcription; Transcription factors; Transcriptome - genetics; cold acclimation; CBF regulons; Arabidopsis; C-repeat binding factor (CBF) transcription factors; chilling response
• ISSN: 0028-646X; 1469-8137
• DOI: 10.1111/nph.14088

In Arabidopsis, the C-repeat binding factors (CBFs) have been extensively studied as key transcription factors in the cold stress response. However, their exact functions in the cold response remains unclear due to the lack of a null cbf triple mutant. In this study, we used CRISPR/Cas9 technology to mutate CBF1 or CBF1/CBF2 in a cbf3 T-DNA insertion mutant to generate cbf1,3 double and cbf1 cbf2 cbf3 (cbfs) triple mutants. The response of the cbfs triple mutants to chilling stress is impaired. Furthermore, no significant difference in freezing tolerance was observed between the wild-type and the cbf1,3 and cbfs mutants without cold acclimation. However, the cbfs mutants were extremely sensitive to freezing stress after cold acclimation, and freezing sensitivity ranking was cbfs > cbf1,3 > cbf3. RNA-Seq analysis showed that 134 genes were CBF regulated, of which 112 were regulated positively and 22 negatively by CBFs. Our study reveals the essential functions of CBFs in chilling stress response and cold acclimation, as well as defines a set of genes as CBF regulon. It also provides materials for the genetic dissection of components in CBF-dependent cold signaling.