Mosquito-borne viruses, insect-specific flaviviruses (family Flaviviridae, genus Flavivirus), Banna virus (family Reoviridae, genus Seadornavirus), Bogor virus (unassigned member of family Permutotetraviridae), and alphamesoniviruses 2 and 3 (family Mesoniviridae, genus Alphamesonivirus) isolated from Indonesian mosquitoes

• Published in: Journal of Veterinary Medical Science Vol. 82; no. 7; pp. 1030 - 1041
• Main Authors: SUPRIYONO, KUWATA, Ryusei, TORII, Shun, SHIMODA, Hiroshi, ISHIJIMA, Keita, YONEMITSU, Kenzo, MINAMI, Shohei, KURODA, Yudai, TATEMOTO, Kango, TRAN, Ngo Thuy Bao, TAKANO, Ai, OMATSU, Tsutomu, MIZUTANI, Tetsuya, ITOKAWA, Kentaro, ISAWA, Haruhiko, SAWABE, Kyoko, TAKASAKI, Tomohiko, YULIANI, Dewi Maria, ABIYOGA, Dimas, HADI, Upik Kesumawati, SETIYONO, Agus, HONDO, Eiichi, AGUNGPRIYONO, Srihadi, MAEDA, Ken
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 2020; Japan Science and Technology Agency; The Japanese Society of Veterinary Science
• Subjects: Aedes; Alphamesonivirus; Animals; Banna virus; Biodiversity; Cell Line; Cell lines; Chlorocebus aethiops; Cricetinae; Culicidae; Culicidae - virology; Flaviviridae; Flavivirus; High-Throughput Nucleotide Sequencing; Indonesia - epidemiology; insect flavivirus; mesonivirus; mosquito; Mosquito Vectors - virology; Mosquitoes; Next-generation sequencing; permutotetravirus; Polymerase chain reaction; Reoviridae; Reverse Transcriptase Polymerase Chain Reaction; Risk assessment; RNA Virus Infections - epidemiology; RNA Viruses - genetics; RNA Viruses - isolation & purification; Surveillance; Vero Cells; Virology; Viruses; Indonesia; mesonivirus; permutotetravirus; insect flavivirus; Banna virus; mosquito
• ISSN: 0916-7250; 1347-7439; 1347-7439
• DOI: 10.1292/jvms.20-0261

Mosquitoes transmit many kinds of arboviruses (arthropod-borne viruses), and numerous arboviral diseases have become serious problems in Indonesia. In this study, we conducted surveillance of mosquito-borne viruses at several sites in Indonesia during 2016–2018 for risk assessment of arbovirus infection and analysis of virus biodiversity in mosquito populations. We collected 10,015 mosquitoes comprising at least 11 species from 4 genera. Major collected mosquito species were Culex quinquefasciatus, Aedes albopictus, Culex tritaeniorhynchus, Aedes aegypti, and Armigeres subalbatus. The collected mosquitoes were divided into 285 pools and used for virus isolation using two mammalian cell lines, Vero and BHK-21, and one mosquito cell line, C6/36. Seventy-two pools showed clear cytopathic effects only in C6/36 cells. Using RT-PCR and next-generation sequencing approaches, these isolates were identified as insect flaviviruses (family Flaviviridae, genus Flavivirus), Banna virus (family Reoviridae, genus Seadornavirus), new permutotetravirus (designed as Bogor virus) (family Permutotetraviridae, genus Alphapermutotetravirus), and alphamesoniviruses 2 and 3 (family Mesoniviridae, genus Alphamesonivirus). We believed that this large surveillance of mosquitoes and mosquito-borne viruses provides basic information for the prevention and control of emerging and re-emerging arboviral diseases.