Study on Evaluation of Alanine Aminotransferase (ALT) as Surrogate Marker in Hepatitis Virus Test

• Published in: Journal of Medical and Dental Sciences Vol. 63; no. 2/3; pp. 45 - 52
• Main Authors: Ikeda, Daisuke, Sugawa, Makiko, Kawahara, Kazuo
• Format: Journal Article
• Language: English
• Published: Japan Tokyo Medical and Dental University 2016
• Subjects: Adult; Alanine Transaminase - blood; Biomarkers - blood; Female; Hepatitis Antibodies - blood; Hepatitis Antigens - blood; Hepatitis B - blood; Hepatitis B - enzymology; Hepatitis B - immunology; Hepatitis B - virology; Hepatitis C - blood; Hepatitis C - enzymology; Hepatitis C - immunology; Hepatitis C - virology; Hepatitis Viruses - isolation & purification; Humans; Male; Middle Aged; Nucleic Acid Amplification Techniques; Regression Analysis; ROC Curve
• ISSN: 1342-8810; 2185-9132
• DOI: 10.11480/jmds.630302

Nucleic acid amplification test (NAT), which was introduced by the Japanese Red Cross Society in October 1999, began to be performed for screening of blood transfusion formulations in Japan in August 2014. In this study, the precision of immunological screenings of hepatitis B (HBsAg, HBcAb, and HBsAb), hepatitis C (HCVAb), and human immunodeficiency (HIVAb) virus antigens in donated blood were evaluated. In addition, the sensitivity of the alanine aminotransferase (ALT) test for detection of the hepatitis B and C viruses was re-evaluated. Immunological screenings showed high precision of detecting the viral antigens. In contrast, the ALT test showed much lower precision of detecting the presence of the hepatitis B and C viruses. Results of the NAT and immunological screenings revealed that ALT levels in donors were more strongly correlated with their levels of gamma-glutamyltranspeptidase (γGTP) and body mass index (BMI), than with the results of NAT and immunological screening. Our study indicates that elevated level(s) of ALT, were more likely to be associated with lifestyles factors such as high intake of alcohol or obesity than with infection. Therefore, ALT may be excluded as surrogate markers of HBV, HCV, and HIV in donated blood.