Time course analysis of RNA quality in placenta preserved by RNAlater or flash freezing

• Published in: American journal of reproductive immunology (1989) Vol. 77; no. 4; pp. np - n/a
• Main Authors: Martin, Nicole M., Cooke, Katherine M., Radford, Caitlin C., Perley, Lauren E., Silasi, Michelle, Flannery, Clare A.
• Format: Journal Article
• Language: English
• Published: Denmark Wiley Subscription Services, Inc 01.04.2017
• Subjects: Cryopreservation - methods; decidua; Female; housekeeping gene; Humans; Organ Preservation Solutions; Placenta; Pregnancy; Real-Time Polymerase Chain Reaction; RNA - analysis; RNA Integrity Number; Time Factors; Tissue Fixation - methods; housekeeping gene; decidua; RNA Integrity Number
• ISSN: 1046-7408; 1600-0897
• DOI: 10.1111/aji.12637

Problem Preservation of biospecimen quality is critical to accurately and reliably assessing genes and proteins. We evaluated the effect of preparation method and storage duration on RNA quality in placenta and decidua. Method of study Aliquots of nine placentas and decidua were placed in RNAlater® (RL) or flash frozen (FF) within 30 minutes of delivery. RNA was extracted immediately (baseline) and from matched samples stored at −80°C for 1 and 8‐10 months. RNA Integrity Number (RIN) and housekeeping gene expression were quantified. Results At both time points, RL placenta had RIN and housekeeping gene Ct values similar to baseline. However, FF placenta had significantly lower RIN and higher Ct values at 1 and 8‐10 months. In RL and FF decidua, RIN was unchanged from baseline. Conclusion We found RNAlater more effectively and consistently preserved placenta, compared to flash freezing. However, for decidua, which is less dense than placenta, both modes yielded comparable RNA integrity over time.