Time course analysis of RNA quality in placenta preserved by RNAlater or flash freezing
Problem Preservation of biospecimen quality is critical to accurately and reliably assessing genes and proteins. We evaluated the effect of preparation method and storage duration on RNA quality in placenta and decidua. Method of study Aliquots of nine placentas and decidua were placed in RNAlater®...
Saved in:
Published in | American journal of reproductive immunology (1989) Vol. 77; no. 4; pp. np - n/a |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Denmark
Wiley Subscription Services, Inc
01.04.2017
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Problem
Preservation of biospecimen quality is critical to accurately and reliably assessing genes and proteins. We evaluated the effect of preparation method and storage duration on RNA quality in placenta and decidua.
Method of study
Aliquots of nine placentas and decidua were placed in RNAlater® (RL) or flash frozen (FF) within 30 minutes of delivery. RNA was extracted immediately (baseline) and from matched samples stored at −80°C for 1 and 8‐10 months. RNA Integrity Number (RIN) and housekeeping gene expression were quantified.
Results
At both time points, RL placenta had RIN and housekeeping gene Ct values similar to baseline. However, FF placenta had significantly lower RIN and higher Ct values at 1 and 8‐10 months. In RL and FF decidua, RIN was unchanged from baseline.
Conclusion
We found RNAlater more effectively and consistently preserved placenta, compared to flash freezing. However, for decidua, which is less dense than placenta, both modes yielded comparable RNA integrity over time. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 MS. NICOLE MICQUETTE MARTIN (Orcid ID : 0000-0002-1955-7509) |
ISSN: | 1046-7408 1600-0897 |
DOI: | 10.1111/aji.12637 |