DEFICIENCY SCREENING FOR GENOMIC REGIONS WITH EFFECTS ON ENVIRONMENTAL SENSITIVITY OF THE SENSORY BRISTLES OF DROSOPHILA MELANOGASTER

• Published in: Evolution Vol. 66; no. 9; pp. 2878 - 2890
• Main Authors: Takahashi, Kazuo H., Okada, Yasukazu, Teramura, Kouhei
• Format: Journal Article
• Language: English
• Published: Malden, USA Wiley Subscription Services, Inc 01.09.2012; Blackwell Publishing Inc; Oxford University Press
• Subjects: Animals; Correlation analysis; Developmental stability; Drosophila; Drosophila melanogaster - genetics; Drosophila melanogaster - growth & development; Environment; environmental sensitivity; Evolution; Evolutionary biology; Evolutionary genetics; Female; fluctuating asymmetry; Genome, Insect; Genomics; Genotype & phenotype; Genotypes; Insect genetics; Insects; isogenic deficiency strains; Male; Phenotype; Phenotypic plasticity; Phenotypic traits; Quantitative genetics; Quantitative trait loci; reaction norm; Ribonucleic acid; RNA; Sensilla
• ISSN: 0014-3820; 1558-5646
• DOI: 10.1111/j.1558-5646.2012.01636.x

Environmental canalization is defined as a reduction in the effect of external environmental perturbations on a phenotype, while phenotypic plasticity is defined as the production of different phenotypes in alternative environments. These terms describe different aspects of the same phenomenon, that is, the sensitivity of the phenotype to the environment. Genetic regulation of the environmental sensitivity has been a central topic in the field of evolutionary biology. In this study, we performed deficiency screening to detect genomic regions with effects on the environmental sensitivity of Drosophila melanogaster sensory bristles. We used a collection of isogenic deficiency strains established by the DrosDel Project for screening. We screened 423 genomic deficiencies that encompassed approximately 63.6% of the entire D. melanogaster genome. We identified 29 genomic deficiencies showing significant effects on environmental sensitivity, suggesting that multiple genomic regions may influence phenotypic variation. We also found significant correlations among the effects of deficiencies on environmental sensitivity for different bristle traits, suggesting that the same genetic mechanism can regulate environmental sensitivity of multiple traits. Current high-resolution mapping will facilitate the examination of individual candidate genes using mutations or RNAi approaches in future studies.