SOME CHARACTERISTICS IN THE PATHWAY OF RNA SYNTHESIS IN HEPATOMA CELLS

The incorporation of labeled uridine into Yoshida ascites hepatoma as well as into 3'-Me-DAB induced primary hepatoma of the rat was found to be extremely higher than that of labeled orotic acid. In normal rat liver and regenerating rat liver, orotic acid was actively utilized for RNA synthesis...

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Published inGANN Japanese Journal of Cancer Research Vol. 59; no. 1; pp. 61 - 71_2
Main Authors MATSUDAIRA, Hiromichi, NAKAMURA, Chiyoko, HISHIZAWA, Tokutaro
Format Journal Article
LanguageEnglish
Published Japan The Japanese Cancer Association 01.02.1968
Subjects
Animals
Ascites
Autoradiography
Carbon Isotopes
Carcinoma, Hepatocellular - chemically induced
Carcinoma, Hepatocellular - metabolism
Liver Neoplasms - chemically induced
Liver Neoplasms - metabolism
Male
Neoplasms, Experimental - chemically induced
Neoplasms, Experimental - metabolism
Nucleotidyltransferases - metabolism
Orotic Acid - metabolism
p-Dimethylaminoazobenzene
Phosphotransferases - metabolism
Rats
RNA, Neoplasm - biosynthesis
Sarcoma, Yoshida - metabolism
Transferases - metabolism
Tritium
Uridine - metabolism
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Summary:The incorporation of labeled uridine into Yoshida ascites hepatoma as well as into 3'-Me-DAB induced primary hepatoma of the rat was found to be extremely higher than that of labeled orotic acid. In normal rat liver and regenerating rat liver, orotic acid was actively utilized for RNA synthesis in contrast to uridine which was poorly utilized in these tissues for RNA synthesis. The increased incorporation of labeled uridine into RNA of the ascites hepatoma cells was found to be not due to increased RNA polymerase activity but due to changes in the mechanisms of precursor uptake, especially to a high uridine kinase activity of the cells.
ISSN:0016-450X
DOI:10.20772/cancersci1959.59.1_61