Anti-HIV-1 Activity of an Ionically Modified Porous Polypropylene Membrane Determined by Filtration of a Viral Suspension

• Published in: MICROBIOLOGY and IMMUNOLOGY Vol. 43; no. 2; pp. 141 - 151
• Main Authors: Owada, Takashi, Miyashita, Yuko, Motomura, Tadahiro, Onishi, Makoto, Yamashita, Shuzo, Yamamoto, Naoki
• Format: Journal Article
• Language: English
• Published: Tokyo Center For Academic Publications Japan 01.01.1999; Center for Academic Publications Japan
• Subjects: Action of physical and chemical agents; AIDS/HIV; Anti-viral activity; Biological and medical sciences; Cell Line; Filtration; Fundamental and applied biological sciences. Psychology; HIV-1 (human immunodeficiency virus type 1); HIV-1 - ultrastructure; Human immunodeficiency virus 1; Humans; Ionically modified membrane; Membranes, Artificial; Microbiology; Microscopy, Electron, Scanning; Polyethyleneimine; Polypropylenes; Virion - ultrastructure; Virology; ζ-Potential; Ethyleneimine derivative polymer; Electric potential; Cationic resin; HIV-1 virus; Propylene polymer; Retroviridae; Artificial membrane; Lentivirus; Biological activity; Virus; Infectivity; Membrane filtration; Antiviral; Human immunodeficiency virus; Inhibition
• ISSN: 0385-5600; 1348-0421
• DOI: 10.1111/j.1348-0421.1999.tb02385.x

We describe here a unique anti-HIV-1 membrane, derived from a chemically modified porous polypropylene (PP) membrane, which lowers viral infectivity upon the filtration of HIV-1 suspension. A cationic polymer, polyethyleneimine (PEI) was graft-polymerized onto the PP filter membrane (PP-PEI), and infectious HIV-1HTLV-IIIB derived from MOLT-4/HIV-1HTLV-IIIB cells (HIV-1HTLV-IIIB(MOLT-4)) was applied. When a viral suspension of high titer (103.93 TCID50 ml-1) was filtered, efficient reduction (>99%) of gag p24 antigen levels and infectious titer resulted. In a viral suspension of medium titer (102.37 TCID50 ml-1), a significant decrease in the p24 antigen did not occur, although the titer was markedly reduced (>95%). Electron microscopic observation suggested that PEI induced viral aggregations under high titer conditions, and under medium titer conditions, PEI deprived HIV-1HTLV-IIIB of its infectivity alone to avoid virus adsorption. In contrast, HIV-1 propagated in human peripheral blood mononuclear cells (PBMC) such as HIV-1HTLV- IIIB(PBMC) was more efficiently trapped by PP-PEI at lower titers as compared with HIV-1HTLV-IIIB(MOLT-4) from MOLT-4/HIV-1HTLV-IIIB cells. These data suggest host cell modification in the interactions between PP-PEI and HIV-1 strains. Since HIV-1HTLV-IIIB(MOLT-4) and HIV-1HTLV-IIIB(PBMC) were almost electrically neutral and negative, respectively, we concluded that the divergent effect of PEI on each HIV-1HTLV-IIIB resulted from their different electrical characteristics.