n-Sec1: A Neural-Specific Syntaxin-Binding Protein
We have identified n-Sec1, a rat brain homolog of the yeast Sec1p protein that participates in the constitutive secretory pathway between the Golgi apparatus and the plasma membrane. The rat brain cDNA is predicted to encode a 68-kDa protein with 65% amino acid identity to Drosophila rop, 59% identi...
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Published in | Proceedings of the National Academy of Sciences - PNAS Vol. 91; no. 4; pp. 1445 - 1449 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Washington, DC
National Academy of Sciences of the United States of America
15.02.1994
National Acad Sciences National Academy of Sciences |
Subjects | |
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Abstract | We have identified n-Sec1, a rat brain homolog of the yeast Sec1p protein that participates in the constitutive secretory pathway between the Golgi apparatus and the plasma membrane. The rat brain cDNA is predicted to encode a 68-kDa protein with 65% amino acid identity to Drosophila rop, 59% identity to Caenorhabditis elegans unc-18, and 27% identity to Saccharomyces cerevisiae Sec1p. By RNA blot analysis, n-Sec1 mRNA expression is neural-specific. An anti-peptide antiserum directed against the n-Sec1 carboxyl terminus detects a 68-kDa protein in rat brain cytosol and membranes, but not in peripheral tissues. In the presence of syntaxin 1a, a plasma membrane protein implicated in synaptic vesicle docking, n-Sec1 becomes membrane-associated. n-Sec1 binds to syntaxin 1a, 2, and 3 fusion proteins coupled to agarose beads, but not to syntaxin 4 fusion protein or beads coupled to a variety of other proteins. These findings indicate that n-Sec1 is a neural-specific, syntaxin-binding protein that may participate in the regulation of synaptic vesicle docking and fusion. |
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AbstractList | A rat brain homolog of the yeast Sec1p protein, which is called neural (n)-Sec1, is identified. The molecule is of particular interest because the yeast syntaxin homologs SSO1 and SSO2 were identified as multicopy suppressors of the sec1 mutation. We have identified n-Sec1, a rat brain homolog of the yeast Sec1p protein that participates in the constitutive secretory pathway between the Golgi apparatus and the plasma membrane. The rat brain cDNA is predicted to encode a 68-kDa protein with 65% amino acid identity to Drosophila rop, 59% identity to Caenorhabditis elegans unc-18, and 27% identity to Saccharomyces cerevisiae Sec1p. By RNA blot analysis, n-Sec1 mRNA expression is neural-specific. An anti-peptide antiserum directed against the n-Sec1 carboxyl terminus detects a 68-kDa protein in rat brain cytosol and membranes, but not in peripheral tissues. In the presence of syntaxin 1a, a plasma membrane protein implicated in synaptic vesicle docking, n-Sec1 becomes membrane-associated. n-Sec1 binds to syntaxin 1a, 2, and 3 fusion proteins coupled to agarose beads, but not to syntaxin 4 fusion protein or beads coupled to a variety of other proteins. These findings indicate that n-Sec1 is a neural-specific, syntaxin-binding protein that may participate in the regulation of synaptic vesicle docking and fusion. We have identified n-Sec1, a rat brain homolog of the yeast Sec1p protein that participates in the constitutive secretory pathway between the Golgi apparatus and the plasma membrane. The rat brain cDNA is predicted to encode a 68-kDa protein with 65% amino acid identity to Drosophila rop, 59% identity to Caenorhabditis elegans unc-18, and 27% identity to Saccharomyces cerevisiae Sec1p. By RNA blot analysis, n-Sec1 mRNA expression is neural-specific. An anti-peptide antiserum directed against the n-Sec1 carboxyl terminus detects a 68-kDa protein in rat brain cytosol and membranes, but not in peripheral tissues. In the presence of syntaxin 1a, a plasma membrane protein implicated in synaptic vesicle docking, n-Sec1 becomes membrane-associated. n-Sec1 binds to syntaxin 1a, 2, and 3 fusion proteins coupled to agarose beads, but not to syntaxin 4 fusion protein or beads coupled to a variety of other proteins. These findings indicate that n-Sec1 is a neural-specific, syntaxin-binding protein that may participate in the regulation of synaptic vesicle docking and fusion. |
Author | Hsu, Shu-Chan Pevsner, Jonathan Scheller, Richard H. |
AuthorAffiliation | Department of Molecular and Cellular Physiology, Howard Hughes Medical Institute, Stanford University Medical Center, CA 94305 |
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Keywords | Binding protein Vertebrata Mammalia Neuron Rat Synaptic transmission Rodentia Neurotransmitter Molecular cloning Aminoacid sequence Structural analysis Brain (vertebrata) |
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Snippet | We have identified n-Sec1, a rat brain homolog of the yeast Sec1p protein that participates in the constitutive secretory pathway between the Golgi apparatus... A rat brain homolog of the yeast Sec1p protein, which is called neural (n)-Sec1, is identified. The molecule is of particular interest because the yeast... We have identified n-Sec1, a rat brain homolog of the yeast Sec1p protein that participates in the constitutive secretory pathway between the Golgi apparatus... |
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SubjectTerms | Amino Acid Sequence Amino acids Analytical, structural and metabolic biochemistry Animals Antibodies Antigens, Surface - metabolism Base Sequence Binding and carrier proteins Biological and medical sciences Blotting, Northern Blotting, Western Brain Brain Chemistry Cell membranes Cell-Free System Cellular biology Cloning, Molecular Complementary DNA Cytosol - chemistry Fundamental and applied biological sciences. Psychology Fungal Proteins - genetics Fungal Proteins - immunology Gels Golgi apparatus Membranes - chemistry Molecular Sequence Data Munc18 Proteins Nerve Tissue Proteins - genetics Nerve Tissue Proteins - metabolism Neurons Peptide Fragments - metabolism Protein Binding Protein Biosynthesis Proteins Rats Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - immunology RNA Rodents Sequence Analysis, DNA Sequence Homology, Amino Acid Syntaxin 1 Tissue Distribution Transcription, Genetic Vesicular Transport Proteins Yeasts |
Title | n-Sec1: A Neural-Specific Syntaxin-Binding Protein |
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