FURTHER CHARACTERIZATION OF A THERMOSENSITIVE TRANSFORMATION VARIANT OF MOUSE FIBROBLASTS

• Published in: GANN Japanese Journal of Cancer Research Vol. 71; no. 6; pp. 775 - 783
• Main Authors: ANDOH, Toshiwo, LIAW, Wen-Shing
• Format: Journal Article
• Language: English
• Published: Japan The Japanese Cancer Association 01.01.1980
• Subjects: Anchorage dependence; Animals; Blood; Cell Transformation, Neoplastic; Cells, Cultured; Culture Media; Endogenous type-C virus; Fibrinolytic activity; Fibrinolytic Agents - secretion; Fibroblasts - microbiology; Fibroblasts - pathology; Fibronectins - analysis; Mice; Mutation; Retroviridae - isolation & purification; Saturation density; Serum requirement; Temperature; Temperature-sensitive variant; Transformation
• ISSN: 0016-450X
• DOI: 10.20772/cancersci1959.71.6_775

Temperature-sensitive (ts) variants that express a transformed phenotype at low (33°) but not at high (38.5°) temperature were isolated from a mouse fibroblast strain C3H2K cells. Among these variants, cloned ts-12B cells showed at 38.5° a density-dependent inhibition of growth typical of normal fibroblasts cultured in vitro. However, at low temperature they lost this capacity and grew to a higher saturation density. The ts variant was also temperature-sensitive as regards serum requirement: it required a higher concentration of serum for growth at 38.5° than at 33°. However, the cells behaved at both temperatures like the parent strain, possessing anchorage-dependence for growth and fibronectin, whereas they were like transformed cells with respect to release of high fibrinolytic activity. No type-C virus core protein p30 was detected at either temperature. Thus, various parameters of transformation in vitro were independently regulated in these variant cells.