Development and Characterization of EST-SSR Markers for Tephroseris furusei (Kitam.) B. Nord. (Asteraceae) and Cross-species Amplification

Twelve polymorphic EST-SSR markers were developed and characterized for Tephroseris furusei (Kitam.) B. Nord., an endangered species endemic to limestone habitats in central Japan, and related spe- cies. In the two T. furusei populations, the total numbers of alleles for each locus ranged from 5 to...

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Published inActa Phytotaxonomica et Geobotanica Vol. 73; no. 1; pp. 87 - 92
Main Authors Mitsui, Yuki, Sato, Chiyoshi, Chiba, Satoshi, Tsuchiyama, Tatsuya, Fuchino, Kento, Asano, Yuki, Tanaka, Keisuke, Miyamoto, Futoshi
Format Journal Article
LanguageEnglish
Published The Japanese Society for Plant Systematics 28.02.2022
Subjects
conservation genetics
endangered plants
EST-SSR
fixation index
genetic diversity
microsatellite
Tephroseris
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Summary:Twelve polymorphic EST-SSR markers were developed and characterized for Tephroseris furusei (Kitam.) B. Nord., an endangered species endemic to limestone habitats in central Japan, and related spe- cies. In the two T. furusei populations, the total numbers of alleles for each locus ranged from 5 to 16 (average = 8.75). The observed (HO) and expected (HE) heterozygosities ranged from 0.33 to 0.76 (average = 0.58) and 0.50 to 0.89 (average = 0.75), respectively. The inbreeding coefficient (fixation index, FIS) ranged from 0.14 to 0.50 (average = 0.24) and five loci deviated significantly from Hardy–Weinberg equi- librium. The versatility of the markers was evaluated for three congenerics: T. flammea (Turcz. ex DC.) Holub subsp. flammea, T. integrifolia (L.) Holub subsp. kirilowii (Turcz. ex DC.), and T. pierotii (Miq.) Holub. All of the EST-SSR markers were transferrable among them.
ISSN:1346-7565
2189-7042
DOI:10.18942/apg.202117