Correlation between blood flow, tissue volume and microvessel density in the flap

• Published in: Nagoya journal of medical science Vol. 82; no. 2; pp. 291 - 300
• Main Authors: Nakamura, Yutaka, Takanari, Keisuke, Nakamura, Ryota, Ono, Masashi, Uchibori, Takafumi, Hishida, Masashi, Murotani, Kenta, Ebisawa, Katsumi, Akagawa, Miki, Kamei, Yuzuru
• Format: Journal Article
• Language: English
• Published: Japan Nagoya University 01.05.2020
• Subjects: Animals; Free Tissue Flaps - blood supply; Free Tissue Flaps - physiology; Free Tissue Flaps - transplantation; Jejunum - blood supply; Jejunum - physiology; Jejunum - transplantation; Microvascular Density; Organ Size; Original Paper; Rabbits; Superficial Back Muscles - blood supply; Superficial Back Muscles - physiology; Superficial Back Muscles - transplantation; Surgical Flaps - blood supply; Surgical Flaps - physiology; Vascular Resistance; microvessel density; free flap transplantation; conductance; blood flow; vessel resistance
• ISSN: 0027-7622; 2186-3326
• DOI: 10.18999/nagjms.82.2.291

The purpose of this study was to assess the correlation between tissue volume and blood flow of the flap in an animal model. Using animal model, tissue volume can be attenuated, and precise change of blood flow could be evaluated. We further investigate the relationship between blood flow and vascular density in the tissue. In this study, we assessed flap conductance (ml/min/mm Hg) as to evaluate the conductivity of blood flow into the flap. Japanese white rabbit was used (n = 7) for this study. The amount of blood flow of jejunal and latissimus dorsi muscle (LD) flaps was measured while removing the distal portion of the flap sequentially. Conductance at each time was calculated from blood pressure and blood flow volume. The tissue volume at each time was also measured. The correlation between conductance and volume was analyzed using a linear mixed model. Immunohistochemical evaluation of microvessel densities (MVD) in these tissues was also performed for CD31/PECAM1 positive area. Conductance and tissue volume were significantly correlated in both jejunal and LD flaps. As the volume increases by 1 cm , the conductance increased significantly by 0.012 ml/min/mm Hg in jejunum, and by 0.0047 ml/min/mm Hg in LD. Mean MVD was 1.15 ± 0.52% in the jejunum and 0.37 ± 0.29% in the LD muscle. In this study, we revealed that flap conductance is proportional to volume and proportional constant is different between the type of tissue. It suggests that the difference of MVD creates the unique conductance of each tissue.