Determination of Acetaldehyde in Human Blood by High-Performance Liquid Chromatography Using Fluorometry

A method was developed for the measurement of acetaldehyde in human blood by high-performance liquid chromatography (HPLC). The method was based on a pre-column reaction; a fluorescent substance was formed by a coupling reaction between 2mol of cyclohexan-1, 3-dione and 1 mol of acetaldehyde with am...

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Published inChemical & pharmaceutical bulletin Vol. 37; no. 1; pp. 200 - 201
Main Authors TAKAYANAGI, Miyuki, GOTO, Shoji, KOKUBO, Yukio, SUZUKI, Makoto, YASHIRO, Tamotsu
Format Journal Article
LanguageEnglish
Published Tokyo The Pharmaceutical Society of Japan 01.01.1989
Maruzen
Japan Science and Technology Agency
Subjects
Acetaldehyde - blood
Alcoholism and acute alcohol poisoning
Biological and medical sciences
Chromatography, High Pressure Liquid
human blood
Humans
Medical sciences
Spectrometry, Fluorescence
Toxicology
Assay
Human
Biological fluid
Ethanol
Metabolite
HPLC chromatography
Blood
Fluorescence spectrometry
Acetaldehyde
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Summary:A method was developed for the measurement of acetaldehyde in human blood by high-performance liquid chromatography (HPLC). The method was based on a pre-column reaction; a fluorescent substance was formed by a coupling reaction between 2mol of cyclohexan-1, 3-dione and 1 mol of acetaldehyde with ammonium acetate. The coupling compound was analyzed by HPLC and the concentration of acetaldehyde was obtained from the calibration curve drawn from the results using standard solutions. The determination was sensitive and reproducible with a range of 0.2-10 μM and a precision (coefficient of variation) of 2.43%.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0009-2363
1347-5223
DOI:10.1248/cpb.37.200