OsCYP2, a chaperone involved in degradation of auxin‐responsive proteins, plays crucial roles in rice lateral root initiation

• Published in: The Plant journal : for cell and molecular biology Vol. 74; no. 1; pp. 86 - 97
• Main Authors: Kang, Bo, Zhang, Zhongchen, Wang, Lingling, Zheng, Libin, Mao, Weihua, Li, Meifei, Wu, Yunrong, Wu, Ping, Mo, Xiaorong
• Format: Journal Article
• Language: English
• Published: England Blackwell Publishing Ltd 01.04.2013
• Subjects: auxin; Cloning, Molecular; cyclophilin; Cyclophilins - genetics; Cyclophilins - metabolism; Gene Expression Regulation, Plant; Indoleacetic Acids - pharmacology; lateral root initiation; Molecular Sequence Data; Mutagenesis; Mutation; Oryza - genetics; Oryza - growth & development; Oryza sativa; Plant Growth Regulators - pharmacology; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Roots - growth & development; Plant sciences; protein interaction; Proteins; Rice; Signal Transduction; Two-Hybrid System Techniques
• ISSN: 0960-7412; 1365-313X
• DOI: 10.1111/tpj.12106

Summary Auxin plays a pivotal role in many facets of plant development. It acts by inducing the interaction between auxin‐responsive [auxin (AUX)/indole‐3‐acetic acid (IAA)] proteins and the ubiquitin protein ligase SCFTIR to promote the degradation of the AUX/IAA proteins. Other cofactors and chaperones that participate in auxin signaling remain to be identified. Here, we characterized rice (Oryza sativa) plants with mutations in a cyclophilin gene (OsCYP2). cyp2 mutants showed defects in auxin responses and exhibited a variety of auxin‐related growth defects in the root. In cyp2 mutants, lateral root initiation was blocked after nuclear migration but before the first anticlinal division of the pericycle cell. Yeast two‐hybrid and in vitro pull‐down results revealed an association between OsCYP2 and the co‐chaperone Suppressor of G2 allele of skp1 (OsSGT1). Luciferase complementation imaging assays further supported this interaction. Similar to previous findings in an Arabidopsis thaliana SGT1 mutant (atsgt1b), degradation of AUX/IAA proteins was retarded in cyp2 mutants treated with exogenous 1‐naphthylacetic acid. Our results suggest that OsCYP2 participates in auxin signal transduction by interacting with OsSGT1.