Effects of Vitamins Added to Culture Medium for Calcified Nodule Formation by Rat Bone Marrow Cells

• Published in: Nano Biomedicine Vol. 15; no. 2; pp. 51 - 65
• Main Authors: NAKAMA, Hitomi, YOSHIKAWA, Masataka, MATSUO, Nozomi, MAEDA, Hiroshi
• Format: Journal Article
• Language: English; Japanese
• Published: Nano Biomedical Society 2023
• Subjects: Bone marrow cells; Calcified nodules; Dexamethasone; Vitamin B
• ISSN: 1883-5198; 2185-4734
• DOI: 10.11344/nano.15.51

The present study attempted to obtain factors that induce the calcified nodule formation in a mesenchymal stem cell (MSC) culture through the aggregation of micro- or nanoparticles, such as calcium carbonate. There are few suitable cell sources in the oral cavity other than dental pulp tissue, from which only a small number of MSCs may be obtained. However, a large number of MSCs are needed for tooth regeneration. Therefore, factors that promote MSC proliferation and differentiation are required. Vitamin B1 (VB1), vitamin B3 (VB3) and vitamin B5 (VB5), which are present in physiologically active substances, were selected and investigated their effects to bone formation in vitro. VB1 and VB3 significantly increased the ability of dexamethasone (Dex) to form calcified nodule aggregates in vitro, whereas VB5 exerted the opposite effects. Therefore, it was confirmed from the results of this in vitro study that VB1 and VB3 may affect to Dex to promote MSCs in rat bone marrow cells for proliferation and differentiation into hard tissue-forming cells and the formation of calcified nodule aggregates.