Enhanced store-operated Ca²+ entry and TRPC channel expression in pulmonary arteries of monocrotaline-induced pulmonary hypertensive rats

• Published in: American Journal of Physiology: Cell Physiology Vol. 302; no. 1; pp. C77 - C87
• Main Authors: Liu, Xiao-Ru, Zhang, Ming-Fang, Yang, Na, Liu, Qing, Wang, Rui-Xing, Cao, Yuan-Ning, Yang, Xiao-Ru, Sham, James S K, Lin, Mo-Jun
• Format: Journal Article
• Language: English
• Published: United States American Physiological Society 01.01.2012
• Subjects: Animals; Calcium; Calcium - metabolism; Calcium - physiology; Calcium homeostasis; Calcium influx; Calcium Signaling - physiology; Cells, Cultured; cyclopiazonic acid; Endothelin 1; Heart; Hypertension; Hypertension, Pulmonary - chemically induced; Hypertension, Pulmonary - metabolism; Hypertrophy; Hypoxia; Lung; Male; Monocrotaline; Monocrotaline - administration & dosage; mRNA; Organ Culture Techniques; Pulmonary artery; Pulmonary Artery - drug effects; Pulmonary Artery - metabolism; Rats; Rats, Sprague-Dawley; Smooth muscle; transient receptor potential proteins; TRPC Cation Channels - biosynthesis; TRPC Cation Channels - physiology; Vascular Biology; Vasoconstriction; Ventricle
• ISSN: 0363-6143; 1522-1563; 1522-1563
• DOI: 10.1152/ajpcell.00247.2011

Pulmonary hypertension (PH) is associated with profound vascular remodeling and alterations in Ca(2+) homeostasis in pulmonary arterial smooth muscle cells (PASMCs). Previous studies show that canonical transient receptor potential (TRPC) genes are upregulated and store-operated Ca(2+) entry (SOCE) is augmented in PASMCs of chronic hypoxic rats and patients of pulmonary arterial hypertension (PAH). Here we further examine the involvement of TRPC and SOCE in PH with a widely used rat model of monocrotaline (MCT)-induced PAH. Rats developed severe PAH, right ventricular hypertrophy, and significant increase in store-operated TRPC1 and TRPC4 mRNA and protein in endothelium-denuded pulmonary arteries (PAs) 3 wk after MCT injection. Contraction of PA and Ca(2+) influx in PASMC evoked by store depletion using cyclopiazonic acid (CPA) were enhanced dramatically, consistent with augmented SOCE in the MCT-treated group. The time course of increase in CPA-induced contraction corresponded to that of TRPC1 expression. Endothelin-1 (ET-1)-induced vasoconstriction was also potentiated in PAs of MCT-treated rats. The response was partially inhibited by SOCE blockers, including Gd(3+), La(3+), and SKF-96365, as well as the general TRPC inhibitor BTP-2, suggesting that TRPC-dependent SOCE was involved. Moreover, the ET-1-induced contraction and Ca(2+) response in the MCT group were more susceptible to the inhibition caused by the various SOCE blockers. Hence, our study shows that MCT-induced PAH is associated with increased TRPC expression and SOCE, which are involved in the enhanced vascular reactivity to ET-1, and support the hypothesis that TRPC-dependent SOCE is an important pathway for the development of PH.