A pair of nonspecific phospholipases C, NPC2 and NPC6, are involved in gametophyte development and glycerolipid metabolism in Arabidopsis

• Published in: The New phytologist Vol. 219; no. 1; pp. 163 - 175
• Main Authors: Ngo, Anh H., Lin, Ying‐Chen, Liu, Yu‐chi, Gutbrod, Katharina, Peisker, Helga, Dörmann, Peter, Nakamura, Yuki
• Format: Journal Article
• Language: English
• Published: England New Phytologist Trust 01.07.2018; Wiley Subscription Services, Inc
• Subjects: Arabidopsis; Arabidopsis - enzymology; Arabidopsis - genetics; Arabidopsis - growth & development; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Arabidopsis thaliana; Bacteria; Body organs; Complementation; Flowers; Flowers - enzymology; Flowers - genetics; Flowers - growth & development; Gametogenesis; gametophyte development; Gametophytes; Germ Cells, Plant - enzymology; Germ Cells, Plant - growth & development; Homeostasis; Hydrolysis; Isoenzymes; Isoforms; Leaves; Lecithin; Lipids; Metabolism; nonspecific phospholipase C; Organs; Ovule - enzymology; Ovule - genetics; Ovule - growth & development; Phosphatidylcholine; Phosphatidylethanolamine; Phosphatidylglycerol; Phospholipase; Phospholipase C; Phospholipases - genetics; Phospholipases - metabolism; phospholipid; Phospholipids; Phospholipids - metabolism; Plant Leaves - enzymology; Plant Leaves - genetics; Plant Leaves - growth & development; Plants (botany); Plants, Genetically Modified; Plastids; Pollen; Promoter Regions, Genetic - genetics; Proteins; Substrates; Type C Phospholipases - genetics; Type C Phospholipases - metabolism; Arabidopsis thaliana; phospholipase; nonspecific phospholipase C; phospholipid; gametophyte development
• ISSN: 0028-646X; 1469-8137
• DOI: 10.1111/nph.15147

Phospholipases play crucial roles in plant membrane lipid homeostasis. Nonspecific phospholipase C (NPCs) establish a unique class of phospholipases found only in plants and certain bacteria. Here, we show that two previously uncharacterized NPC isoforms, NPC2 and NPC6, are required for male and female gametophyte development in Arabidopsis. Double mutant plants of npc2-1 npc6-2 could not be retrieved because npc2-1 npc6-2 ovule and pollen development is affected. Genetic complementation, reciprocal crossing and microscope observation of npc2-1/– npc6-2/+ and npc2-1/+ npc6-2/– plants suggest that NPC2 and NPC6 are redundant and are required for normal gametophyte development. Both NPC2 and NPC6 proteins are localized to the plastids. Promoter-GUS assays in transgenic Arabidopsis revealed that NPC2 and NPC6 are preferentially expressed in floral organs rather than in leaves. In vitro enzyme assays showed that NPC2 and NPC6 hydrolyze phosphatidylcholine and phosphatidylethanolamine, but not phosphatidate, being consistent with the reported substrate selectivity of NPCs. The amounts of phosphatidylcholine, phosphatidylethanolamine and phosphatidylglycerol were increased in buds but not in flowers of npc2-1/– npc6-2/+ and npc2-1/+ npc6-2/– plants, presumably due to reduced phospholipid hydrolysis activity in developing flowers. Our results demonstrate that NPC2 and NPC6 play crucial roles in gametogenesis during flower development.