Characterization of two insertion sequences, IS701 and IS702, from the cyanobacterium Calothrix species PCC 7601

We describe the characterization of two insertion elements, IS701 and IS702, isolated from Calothrix species PCC 7601. These insertion elements were cloned from spontaneous pigmentation mutants. Both show the characteristics of typical bacterial insertion sequences, i.e. they present long terminal i...

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Bibliographic Details
Published inMolecular microbiology Vol. 5; no. 9; p. 2165
Main Authors Mazel, D, Bernard, C, Schwarz, R, Castets, A M, Houmard, J, Tandeau de Marsac, N
Format Journal Article
LanguageEnglish
Published England 01.09.1991
Subjects
Amino Acid Sequence
Base Sequence
Cloning, Molecular
Cyanobacteria - genetics
DNA Transposable Elements - genetics
Gene Library
Molecular Sequence Data
Multigene Family
Mutation
Phycocyanin - genetics
Phycoerythrin - genetics
Sequence Homology, Nucleic Acid
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Summary:We describe the characterization of two insertion elements, IS701 and IS702, isolated from Calothrix species PCC 7601. These insertion elements were cloned from spontaneous pigmentation mutants. Both show the characteristics of typical bacterial insertion sequences, i.e. they present long terminal inverted repeats and they duplicate target DNA upon insertion. These elements share no homology with the only other cyanobacterial insertion sequence described so far, IS891. At least 15 copies of IS701 and 9 copies of IS702 were detected by hybridization experiments in the Calothrix 7601 genome. Their occurrence in several cyanobacterial strains is also reported.
ISSN:0950-382X
DOI:10.1111/j.1365-2958.1991.tb02146.x