Nanoneedle technology to investigate cytoskeletal proteins in living cells

We developed a method to detect intracellular cytoskeletal proteins using antibody-functionalized nanoneedle. The nanoneedles are ultra-thin cylindrical-shaped AFM probes sharpened to 200 nm in diameter. Following insertion, the nanoneedle was retracted from the live cell and the mechanical force ne...

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Bibliographic Details
Published inTransactions of Japanese Society for Medical and Biological Engineering Vol. 52; no. Supplement; pp. SY-39 - SY-40
Main Author Nakamura, Chikashi
Format Journal Article
LanguageJapanese
Published Japanese Society for Medical and Biological Engineering 2014
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Summary:We developed a method to detect intracellular cytoskeletal proteins using antibody-functionalized nanoneedle. The nanoneedles are ultra-thin cylindrical-shaped AFM probes sharpened to 200 nm in diameter. Following insertion, the nanoneedle was retracted from the live cell and the mechanical force needed to unbind the antibody-target protein complex was measured, we named the peak force “fishing force”. Using this method, intermediate filament proteins, actin filaments and microtubules were successfully detected. The intermediate filament proteins, nestin and neurofilament, which are well-known marker proteins of neural cells could be detected in single living cells, mouse embryonic carcinoma P19, within a minute.
ISSN:1347-443X
1881-4379
DOI:10.11239/jsmbe.52.SY-39