Biosynthesis of Polyketomycin Produced by Streptomyces diastatochromogenes Tü 6028

• Published in: Journal of antibiotics Vol. 52; no. 2; pp. 96 - 101
• Main Authors: PAULULAT, THOMAS, ZEECK, AXEL, GUTTERER, JAN MIRKO, FIEDLER, HANS-PETER
• Format: Journal Article
• Language: English
• Published: TOKYO JAPAN ANTIBIOTICS RESEARCH ASSOCIATION 01.02.1999; JAPAN ANTIBIOT RES ASSN; Japan Antibiotics Research Association
• Subjects: Aminoglycosides; Anti-Bacterial Agents - biosynthesis; Anti-Bacterial Agents - isolation & purification; Antibacterial agents; Antibiotics (antibacterial agents, antifungal agents); Antibiotics, microbial producers, chemotherapic agents, antiseptics, disinfecting agents; Antibiotics. Antiinfectious agents. Antiparasitic agents; Applied microbiology; Biological and medical sciences; Biotechnology & Applied Microbiology; Fermentation; Fundamental and applied biological sciences. Psychology; Glyoxylates - isolation & purification; Glyoxylates - metabolism; Immunology; Life Sciences & Biomedicine; Magnetic Resonance Spectroscopy; Medical sciences; Microbiology; Molecular Structure; Pharmacology & Pharmacy; Pharmacology. Drug treatments; Science & Technology; Streptomyces - metabolism; X-Ray Diffraction; POLYKETIDE; Streptomycetaceae; Quinone; Aglycone; Biosynthesis; Tetracyclic compound; Fermentation; In vitro; Actinomycetales; Antibiotic; Medium effect; Microorganism culture; Bacteria; Actinomycetes; Glycoside; Antibacterial agent
• ISSN: 0021-8820; 1881-1469
• DOI: 10.7164/antibiotics.52.96

The biosynthesis of polyketomycin was investigated by feeding 13C-labeled acetate and propionate to the growing cultures of Streptomyces diastatochromogenes Tü 6028. 13C NMR spectral analysis demonstrated the polyketide origin of the aglycone and the dimethylsalicyloyl moieties. The O-methyl group and 6-CH3 of the aglycone as well as 3B-CH3 of L-axenose and 3C-CH3 of the salicyloyl residue were labeled by feeding L-[methyl-13C]methionine. Both deoxysugars emerged from D-glucose. The biosynthesis of the aglycone and the assembly of the glycoside are discussed. The polyketomycin producing strain may be a candidate for further exploration in combinatorial biosynthesis.