Agrobacterium-mediated transformation: rice transformation

Agrobacterium is a common soil bacterium with natural capacity for trans-kingdom transfer of genetic information by transferring its T-DNA into the eukaryotic genome. In agricultural plant biotechnology, combination of non-phytopathogenic strain of Agrobacterium tumefaciens with modified T-DNA and v...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1099; p. 261
Main Authors Slamet-Loedin, Inez H, Chadha-Mohanty, Prabhjit, Torrizo, Lina
Format Journal Article
LanguageEnglish
Published United States 01.01.2014
Subjects
Agrobacterium - genetics
Agrobacterium - metabolism
Gene Transfer Techniques
Oryza - genetics
Oryza - microbiology
Plants, Genetically Modified
Seeds - genetics
Seeds - microbiology
Transformation, Genetic
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Summary:Agrobacterium is a common soil bacterium with natural capacity for trans-kingdom transfer of genetic information by transferring its T-DNA into the eukaryotic genome. In agricultural plant biotechnology, combination of non-phytopathogenic strain of Agrobacterium tumefaciens with modified T-DNA and vir-genes in a binary vector system is the most widely utilized system for genetic improvement in diverse plant species and for gene function validation. Here we have described a highly efficient A. tumefaciens-mediated transformation system for indica and japonica rice cultivars based on an immature embryo system.
ISSN:1940-6029
DOI:10.1007/978-1-62703-715-0_21