Effects of Minor Components of Crude Vegetable Oil on the Enzymatic Method to Analyze Positional Fatty Acid Distributions in Triacylglycerols with Candida antarctica Lipase B

Crude soybean and rapeseed oils were subjected to the method to determine FA distributions in TAG using Candida antarctica lipase B, giving similar results to those for refined oils. Minor components in crude oils, such as percentages of FFA or phospholipids were indicated not to affect 1(3)-selecti...

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Bibliographic Details
Published inJournal of oleo science Vol. 65; no. 11; p. 923
Main Authors Hori, Ryuji, Sano, Takashi, Imagi, Jun, Watanabe, Yomi
Format Journal Article
LanguageEnglish
Published Tokyo Japan Science and Technology Agency 2016
Subjects
Enzyme activity
Fatty acids
Lipase
Transesterification
Triglycerides
Vegetable oils
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Summary:Crude soybean and rapeseed oils were subjected to the method to determine FA distributions in TAG using Candida antarctica lipase B, giving similar results to those for refined oils. Minor components in crude oils, such as percentages of FFA or phospholipids were indicated not to affect 1(3)-selective transesterification by the lipase and FA compositional analysis of the resulting 2-MAG fraction significantly. Phospholipids were confirmed not to contaminate the 2-MAG fraction. Oxidized soybean oil with a PV of 10 meq/kg also gave similar results to the ones for refined oil. The method was confirmed to be applicable for crude oils and oxidized oils with a PV smaller than 10 meq/kg without prior purification of TAG.
ISSN:1345-8957
1347-3352
DOI:10.5650/jos.ess16106