Regeneration of Bone Tissue in a Controlled in Vitro Environment with a Rotating Wall Vessel Bioreactor

• Published in: Nano Biomedicine Vol. 3; no. 2; pp. 267 - 274
• Main Authors: NISHI, Masanori, MATSUMOTO, Rena, DONG, Jian, UEMURA, Toshimasa
• Format: Journal Article
• Language: English
• Published: Nano Biomedical Society 2011
• Subjects: Biocompatibility; Biomedical materials; Bioreactors; Bone marrow; bone marrow cell; Bones; culture environment; In vitro testing; mesenchcymal stem cell; Nanostructure; Rotating; rotating wall vessel bioreactor; three-dimensional culture
• ISSN: 1883-5198; 2185-4734
• DOI: 10.11344/nano.3.267

To generate mature bone tissue in vitro, many types of cells in rabbit bone marrow aspirate were cultured within a rotating wall vessel (RWV) bioreactor. Bone marrow aspirate contains not only mesenchymal stem cells (MSCs) but also hematopoietic stem cells (HSCs), osteoblasts, osteocytes, osteoclasts, etc. Non-adherent cells in bone marrow aspirate were co-cultured with MSCs and MSC-derived endothelial cells under rotating condition with an RWV bioreactor. The engineered tissue was analyzed histochemically, and high-density tissue, a pre-bone-like structure containing osteoclast-like cells and thick vascular-like structures (approximately 50μm in diameter) were observed. Moreover, MSC-derived osteoblasts were co-cultured with these cells, and osteogenesis of cultured tissue was accelerated. Many types of cells and growth factors can repeatedly interact with each other in a rotating culture with an RWV bioreactor. These conditions could provide an excellent controlled environment for constructing bone tissue in vitro.