Modular Surface Display for Porcine Circovirus Type 2 Virus-like Particles Mediated by Microbial Transglutaminase

Virus-like particles (VLPs) are multi-protein complex, which mimic viral particles without viral genomes. Recently various applications of VLPs for medical and pharmaceutical fields are developed. Furthermore, surface modification of VLPs is expected to extend their functional versatility. As an enz...

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Published inJournal of Insect Biotechnology and Sericology Vol. 87; no. 2; pp. 2_053 - 2_060
Main Authors Masuda, Akitsu, Minamihata, Kosuke, Hino, Masato, Morokuma, Daisuke, Karasaki, Noriko, Mon, Hiroaki, Kamiya, Noriho, Kusakabe, Takahiro, Lee, Jae Man
Format Journal Article
LanguageEnglish
Published Ibaraki The Japanese Society of Sericultural Science 2018
Japan Science and Technology Agency
Subjects
Baculoviridae
Baculovirus
C-Terminus
Cadaverine
Capsid protein
Covalent bonds
Crosslinking
Display devices
Genomes
Glutamine
Immunogenicity
Lysine
Microbial transglutaminase
Microorganisms
N-Terminus
Porcine circovirus type 2
Proteins
Silkworm-baculovirus expression vector system
Silkworms
Surface modification
Vaccines
Virus-like particles
Viruses
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Summary:Virus-like particles (VLPs) are multi-protein complex, which mimic viral particles without viral genomes. Recently various applications of VLPs for medical and pharmaceutical fields are developed. Furthermore, surface modification of VLPs is expected to extend their functional versatility. As an enzymatic strategy for the modification, microbial transglutaminase (MTG) that catalyzes the covalent bond between a glutamine residue and a lysine residue or a primary amine is suitable for efficient protein ligation. In the previous study, we demonstrated the efficient production of immunogenic VLPs of porcine circovirus type 2 (PCV2) using silkworm-baculovirus expression vector system (silkworm-BEVS). Herein, we established the display system of exotic molecules to VLPs by enzymatic covalent cross-linking using MTG. For the target modification, Q-tag (YPLQMRG) that is MTG reactive sequence were introduced into the N-terminus, C-terminus, or loop BC of capsid protein of PCV2 and successfully expressed as VLPs in silkworm pupae. Of these, PCV2 VLPs with Q-tag at the loop BC and C-terminus were efficiently conjugated with FITC-cadaverine and K-tagged (MRHKGS) EGFP by MTG. These results showed that flexible surface modification of VLPs mediated by MTG could be used for development of several therapeutic tools such as multivalent vaccines.
ISSN:1346-8073
1884-7978
DOI:10.11416/jibs.87.2_053