Application of Mild Acetolysis to Confirm the Comb-like Structure of Cell Wall Mannan from Pichia pastoris IFO 0948 Strain

• Published in: Chemical & pharmaceutical bulletin Vol. 36; no. 8; pp. 3168 - 3172
• Main Authors: KOBAYASHI, HIDEMITSU, SHIBATA, NOBUYUKI, YONEZU, TOSHIO, SUZUKI, SHIGEO
• Format: Journal Article
• Language: English
• Published: Tokyo The Pharmaceutical Society of Japan 25.08.1988; Maruzen
• Subjects: Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Microbiology; Morphology, structure, chemical composition; Mycology; Pichia pastoris; β-linkage mannan; Fungi; Investigation method; Acetolysis; Ascomycetes; Mannan; Cell wall; Thallophyta

In order to confirm the chemical structure of the mannan of Pichia pastoris IFO 0948 strain, which was assumed to possess a highly branched comb-like structure, a sequential degradation procedure involving partial acid hydrolysis followed by acetolysis under mild conditions was employed. This proced...