Application of Mild Acetolysis to Confirm the Comb-like Structure of Cell Wall Mannan from Pichia pastoris IFO 0948 Strain

In order to confirm the chemical structure of the mannan of Pichia pastoris IFO 0948 strain, which was assumed to possess a highly branched comb-like structure, a sequential degradation procedure involving partial acid hydrolysis followed by acetolysis under mild conditions was employed. This proced...

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Bibliographic Details
Published inChemical & pharmaceutical bulletin Vol. 36; no. 8; pp. 3168 - 3172
Main Authors KOBAYASHI, HIDEMITSU, SHIBATA, NOBUYUKI, YONEZU, TOSHIO, SUZUKI, SHIGEO
Format Journal Article
LanguageEnglish
Published Tokyo The Pharmaceutical Society of Japan 25.08.1988
Maruzen
Subjects
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Microbiology
Morphology, structure, chemical composition
Mycology
Pichia pastoris
β-linkage mannan
Fungi
Investigation method
Acetolysis
Ascomycetes
Mannan
Cell wall
Thallophyta
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Summary:In order to confirm the chemical structure of the mannan of Pichia pastoris IFO 0948 strain, which was assumed to possess a highly branched comb-like structure, a sequential degradation procedure involving partial acid hydrolysis followed by acetolysis under mild conditions was employed. This procedure can widely be adopted as a method for determining the structural shape of fungal mannans possessing a highly branched structure, either comb-like or tree-like, without application of enzymolytic degradation.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0009-2363
1347-5223
DOI:10.1248/cpb.36.3168