COMPARISON OF THE DYE TEST AND HEMAGGLUTINATION TESTS BY THREE DIFFERENT TECHNIQUES IN THE SEROLOGIC DIAGNOSIS OF TOXOPLASMOSIS RESULTS ON SERA FROM HUMANS, DOGS AND CATS

• Published in: Japanese Journal of Medical Science and Biology Vol. 24; no. 2; pp. 115 - 124
• Main Authors: KUMADA, MITSUYOSHI, KOBAYASHI, AKIO, TSUNEMATSU, YUKINORI, KAMEI, KISEKO, NOBUTO, KENZO, HANAKI, TAKUMA
• Format: Journal Article
• Language: English
• Published: Japan National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee 1971
• Subjects: Animals; Antibodies - analysis; Cat Diseases - diagnosis; Cats; Coloring Agents; Dog Diseases - diagnosis; Dogs; Hemagglutination Tests; Humans; Immune Sera; Methods; Toxoplasmosis - diagnosis; Toxoplasmosis - immunology; Toxoplasmosis, Animal - diagnosis
• ISSN: 0021-5112; 1884-2828
• DOI: 10.7883/yoken1952.24.115

The dye and three different hemagglutination tests for toxoplasmosis were compared and evaluated in regard to the sensitivity and specificity on a total of 228 sera from humans, dogs and cats. Good agreements were obtained among the positive rates by the dye test, a modified Jacobs-Lunde's and the Lewis-Kessel' s hemagglutination tests. Such a high agreement rate as 94% was shown on human sera by these hemagglutination tests, but slightly lower rates, 88.5% and 82.8%, on dog sera. The dye test demonstrated the antibody in all sera from proven cases of toxoplasmosis (29/29), whereas both Jacobs-Lunde's and Lewis-Kessel's hemagglutination tests in 97% (28/29) . Higher titers were demonstrated on the sera by these hemagglutination tests than by the dye test. By Jacobs-Lunde's hemagglutination test, the titer was 2 to 3 tubes (16 to 64 times) higher than that by the dye test. The titer by Lewis-Kessel's technique was 4 tubes (256 times) higher than that by the dye test. The Hanaki-Nobuto-Sato's hemagglutination test was slightly inferior to the other tests although the pre-sensitized and lyophilized erythrocytes are available for the test.