Biosynthesis of Vitamin B12: Factor IV, a New Intermediate in the Anaerobic Pathway

The structure of a novel tetradehydrocorrin, factor IV, isolated from Propionibacterium shermanii has been established by multidimensional NMR spectroscopy. Incorporation of radiolabeled factor IV into cobyrinic acid established the biointermediacy of this cobalt complex, whose structure has implica...

Full description

Saved in:
Bibliographic Details
Published inProceedings of the National Academy of Sciences - PNAS Vol. 93; no. 25; pp. 14316 - 14319
Main Authors Scott, A. Ian, Stolowich, Neal J., Wang, Jianji, Gawatz, Oliver, Fridrich, Erwin, Muller, Gerhard
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences of the United States of America 10.12.1996
National Acad Sciences
National Academy of Sciences
The National Academy of Sciences of the USA
Subjects
Anaerobiosis
Bioconversion
Biosynthesis
Calcium - isolation & purification
Calcium - metabolism
Carboxylates
Cobalt
Esters
Magnetic Resonance Spectroscopy
Oxygen
Physical Sciences
Propionibacterium - metabolism
Protons
Vitamin B 12 - biosynthesis
Vitamin B12
Online AccessGet full text

Cover

More Information
Summary:The structure of a novel tetradehydrocorrin, factor IV, isolated from Propionibacterium shermanii has been established by multidimensional NMR spectroscopy. Incorporation of radiolabeled factor IV into cobyrinic acid established the biointermediacy of this cobalt complex, whose structure has implications for the mechanisms of the anaerobic pathway to B12.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
D. H. R. Barton, Texas A&M University, College Station, TX
To whom reprint requests should be addressed. e-mail: Scott@chemvx.tamu.edu.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.93.25.14316