光線力学療法の創傷治癒効果についての検討

• Published in: Transactions of Japanese Society for Medical and Biological Engineering Vol. 54Annual; no. 28PM-Abstract; p. S337
• Main Authors: 江南, 慧, 寺原, 航, 小島, 涼, 仲谷, 将隆, 萩沢, 康介, 岩屋, 啓一, 守本, 祐司, 吉田, 健一, 鬼木, 玲奈, 長井, 健一郎, 四ノ宮, 成祥, 堀内, 俊克
• Format: Journal Article
• Language: Japanese
• Published: 公益社団法人 日本生体医工学会 2016; Japanese Society for Medical and Biological Engineering
• ISSN: 1347-443X; 1881-4379
• DOI: 10.11239/jsmbe.54Annual.S337

We are exploring the potential use of photodynamic therapy (PDT) for wound healing. In this study, we examined whether PDT with aminolevulinic acid (ALA) promoted epithelization using a skin defect model mouse (C57/BL6, 8-10w, ♂). Mice were applied with ALA gel (10%, 1%, 0.1% 0.01% and 0.0001%) on the skin lesions and irradiated with a 630 nm light source. Skin defect areas were measured on 0, 1, 3, 5, 7, 10, 12 and 14 days after PDT. Fluorometric analysis revealed that red fluorescence probably due to protoporphyrin IX (PPIX), a metabolite of ALA was seen in the ALA applied region. Epithelization in mice with ALA-PDT was significantly faster than that in control mice and angiogenesis was substantially seen in the healed area. These results suggested that PDT using ALA promoted wound healing.