Rapid Screening System for Paralytic Shellfish Toxins in Bivalves by Oligonucleotide Lateral Flow Immunoassay

• Published in: Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) Vol. 62; no. 3; pp. 85 - 93
• Main Authors: Ueno, Ken-ichi, Hosokawa, Aoi, Hashimoto, Satoshi, Oikawa, Hiroshi, Shibahara, Yusuke, Matsushima, Ryoji, Watanabe, Ryuichi, Uchida, Hajime, Suzuki, Toshiyuki
• Format: Journal Article
• Language: Japanese
• Published: Tokyo Japanese Society for Food Hygiene and Safety 25.06.2021; Japan Science and Technology Agency
• Subjects: alternative method to animal experiment; Animal welfare; Bio-assays; Bioassays; Bivalvia; ELISA; Enzyme-linked immunosorbent assay; Experiments; High-performance liquid chromatography; HPLC; Immunoassay; Immunoassays; lateral flow immunoassay mouse bioassay (MBA); Liquid chromatography; Mollusks; Oligonucleotides; paralytic shellfish toxins (PSTs); Patinopecten yessoensis (scallop); Receptors; Screening; Shellfish; Toxins; Japan
• ISSN: 0015-6426; 1882-1006
• DOI: 10.3358/shokueishi.62.85

The mouse bioassay (MBA) for paralytic shellfish toxins (PSTs) in bivalves has been used as an official method in Japan. It is necessary to develop an alternative method to animal experiments in PSTs assay because 3Rs (Replacement, Reduction, and Refinement) of animal experiments are required from the animal welfare point of view. Various methods such as HPLC-FL, receptor binding assay, LC-MS/MS and ELISA have been established to detect PSTs without performing animal experiments. The present study was undertaken to develop a screening method using oligonucleotide lateral flow immunoassay (OLFIA) for detecting PSTs in bivalves. The screening level was defined as positive at 2 MU/g of MBA that is the half regulation limit of PSTs monitoring in Japan. All 20 positive (equal to or more than 2 MU/g) samples judged from MBA showed a positive reaction in the OLFIA. No positive samples resulted in a false negative reaction. The OLFIA exhibited high accuracy at 2 MU/g of screening criteria. The authors demonstrated here that the OLFIA can be useful for rapid detection of PSTs in bivalves.