Molecular detection, community structure and phylogeny of ericoid mycorrhizal fungi

Through traditional culturing and molecular characterization, we have determined that five putative species and 2 polyphyletic assemblages of fungi produce ericoid mycorrhizae in Gaultheria shallon, other Ericaceae and Epacridaceae. Using phylogenetic analysis of ITS2 sequences in GenBank, we have c...

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Published inPlant and soil Vol. 244; no. 1/2; pp. 55 - 66
Main Authors Berch, S.M., Allen, T.R., Berbee, M.L.
Format Journal Article Conference Proceeding
LanguageEnglish
Published Dordrecht Kluwer Academic Publishers 01.07.2002
Springer
Springer Nature B.V
Subjects
Ascomycota
Basidiomycota
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Fungi
Mycorrhizae
Mycorrhizal fungi
Plant roots
Plants
Polymerase chain reaction
Ribosomal DNA
Taxa
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Summary:Through traditional culturing and molecular characterization, we have determined that five putative species and 2 polyphyletic assemblages of fungi produce ericoid mycorrhizae in Gaultheria shallon, other Ericaceae and Epacridaceae. Using phylogenetic analysis of ITS2 sequences in GenBank, we have confirmed that most of these fungi occur in North America, Europe, and Australia. The low recovery rate of culturable ericoid mycorrhizal fungi from Gaultheria shallon may partly be explained by the fact that most mycorrhizal root segments contain an unculturable basidiomycete, revealed by direct amplification, cloning, and sequencing of LSU fungal DNA from root. Molecular characterization and phylogenetic analysis are powerful tools in revealing the geographic distribution and identity of ericoid mycorrhizal fungi.
Bibliography:ObjectType-Article-2
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ISSN:0032-079X
1573-5036
DOI:10.1023/A:1020291516884