ヒト体表のバイオフォトン画像及び分光分析による酸化ストレス計測法の検討

• Published in: Transactions of Japanese Society for Medical and Biological Engineering Vol. Annual56; no. Proc; pp. 7 - 8
• Main Authors: 岩佐, 琥偉, 小林, 正樹
• Format: Journal Article
• Language: Japanese
• Published: 公益社団法人 日本生体医工学会 2018; Japanese Society for Medical and Biological Engineering
• Subjects: Biophoton; Delayed Luminescence; Oxidative stress
• ISSN: 1347-443X; 1881-4379
• DOI: 10.11239/jsmbe.Annual56.7

Biophoton, which is ultraweak photon emission from all living organisms, is known to originate in the production of reactive oxygen species (ROS), generated in the process of energy metabolism and exogenously-induced oxidative stress of living body. On the other hand, living materials show ultraweak light emission after light illumination, known as delayed luminescence (DL), which is long lifetime photon emission continuing several tens of minutes in the dark. It is also partially associated with the production of ROS through photosensitization process. We have developed the system for imaging and spectroscopic analysis of these ultraweak photon emission phenomena, based on a highly sensitive cooled CCD (charge-coupled device) camera and a high throughput lens system. We have measured and compared the properties of biophoton and DL induced by blue LED (light-emitting diode) light using human fingers. The study for elucidation of the mechanism of both photon emission processes and exploration of the feasibility for determination of oxidative stress of living body will be discussed.