Correlation between RsaI restriction fragment length polymorphism and electrophoretic types of human placental alkaline phosphatase

Restriction fragment length polymorphism (RFLP) of human alkaline phosphatases was studied in a population sample from northern Sweden using a placental alkaline phosphatase (PLAP) cDNA probe. After digestion of human genomic DNA with RsaI the Southern blots showed DNA fragments most probably derive...

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Bibliographic Details
Published inHuman heredity Vol. 39; no. 1; p. 41
Main Authors Beckman, G, Beckman, L, Lundgren, E, Millán, J L, Sikström, C
Format Journal Article
LanguageEnglish
Published Switzerland 1989
Subjects
Alkaline Phosphatase - genetics
Alleles
Blotting, Southern
Deoxyribonucleases, Type II Site-Specific - metabolism
DNA Probes
Electrophoresis, Starch Gel
Gene Frequency
Humans
Mutation
Phenotype
Placenta - enzymology
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length
Restriction Mapping
Sweden
Sweden
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Summary:Restriction fragment length polymorphism (RFLP) of human alkaline phosphatases was studied in a population sample from northern Sweden using a placental alkaline phosphatase (PLAP) cDNA probe. After digestion of human genomic DNA with RsaI the Southern blots showed DNA fragments most probably derived from three genes: PLAP, germ cell alkaline phosphatase (PLAP-like) and intestinal alkaline phosphatase. In agreement with a previous study, a two-allele polymorphism was found in PLAP with bands at 1.6 kilobases (A1) and 1.8 kilobases (A2). The gene frequencies of A1 and A2 were 0.46 and 0.54, respectively. There was a significant correlation between the RsaI RFLPs and electrophoretic types of PLAP; RSAI A2 showed an association with the ALP2p allele of PLAP.
ISSN:0001-5652
DOI:10.1159/000153830