A NEW SUBSTRAIN DERIVED FROM HEP-2 FOR THE DETECTION OF ANTI-NUCLEAR ANTIBODIES

• Published in: TISSUE CULTURE RESEARCH COMMUNICATIONS Vol. 17; no. 3; pp. 95 - 100
• Main Authors: TSUBOI, Isami, TAGUCHI, Michihiro, KENMOTSU, Masashi, BAN, Fumihiko, MACHIDA, Kunimitu, INAGAKI, Kouichi, ISHI, Akira, TAZAWA, Tadashi
• Format: Journal Article
• Language: English
• Published: The Japanese Tissue Culture Association 1998
• Subjects: anti-nuclear antibody; cytoplasmic staining pattern; HEp-2; large cell; large nuclei; substrain
• DOI: 10.11418/jtca1981.17.3_95
• ISSN: 0912-3636

A new substrain of cells, named Ban, was obtained from the human epidermoid carcinoma 2 (HEp-2) cell line. The Ban cells, which are larger than HEp-2 cells were isolated using a cell sorter after seeding HEp-2 cells by the limited dilution method. Not only the cell size but also the size of the nucleus of Ban cells was found to be larger than that of HEp-2 cells, and these characteristics were maintained for more than 7 months (46 passages). The chromosome frequency distribution of Ban cells was about 1.5 times greater than that of HEp-2 cells. When cell number were examined comparing Ban cells (50 passages) and HEp-2 cells, no significant difference in cell growth was observed for up to 7 days. Ban cells are useful for detection of anti-nuclear antibodies since these cells display 5 kinds of nuclear staining patterns as in the case of HEp-2 cells. However, the relative fluorescence intensity of Ban cells was greater than that of HEp-2 cells upon staining with anti-cytoplasmic antibodies.