A NEW SUBSTRAIN DERIVED FROM HEP-2 FOR THE DETECTION OF ANTI-NUCLEAR ANTIBODIES

A new substrain of cells, named Ban, was obtained from the human epidermoid carcinoma 2 (HEp-2) cell line. The Ban cells, which are larger than HEp-2 cells were isolated using a cell sorter after seeding HEp-2 cells by the limited dilution method. Not only the cell size but also the size of the nucl...

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Bibliographic Details
Published inTISSUE CULTURE RESEARCH COMMUNICATIONS Vol. 17; no. 3; pp. 95 - 100
Main Authors TSUBOI, Isami, TAGUCHI, Michihiro, KENMOTSU, Masashi, BAN, Fumihiko, MACHIDA, Kunimitu, INAGAKI, Kouichi, ISHI, Akira, TAZAWA, Tadashi
Format Journal Article
LanguageEnglish
Published The Japanese Tissue Culture Association 1998
Subjects
anti-nuclear antibody
cytoplasmic staining pattern
HEp-2
large cell
large nuclei
substrain
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Summary:A new substrain of cells, named Ban, was obtained from the human epidermoid carcinoma 2 (HEp-2) cell line. The Ban cells, which are larger than HEp-2 cells were isolated using a cell sorter after seeding HEp-2 cells by the limited dilution method. Not only the cell size but also the size of the nucleus of Ban cells was found to be larger than that of HEp-2 cells, and these characteristics were maintained for more than 7 months (46 passages). The chromosome frequency distribution of Ban cells was about 1.5 times greater than that of HEp-2 cells. When cell number were examined comparing Ban cells (50 passages) and HEp-2 cells, no significant difference in cell growth was observed for up to 7 days. Ban cells are useful for detection of anti-nuclear antibodies since these cells display 5 kinds of nuclear staining patterns as in the case of HEp-2 cells. However, the relative fluorescence intensity of Ban cells was greater than that of HEp-2 cells upon staining with anti-cytoplasmic antibodies.
ISSN:0912-3636
1881-3704
DOI:10.11418/jtca1981.17.3_95