各種肝疾患における血中免疫複合体の臨床的意義およびB型肝疾患におけるHBs抗原,HBe抗原含有免疫複合体の同定について
Raji cell assayを用いて各種肝疾患における血中免疫複合体(CIC)の出現頻度,疾患活動性との関連について検討し,さらにB型肝疾患についてはPEG-trypsinisation assayによりHBs抗原,HBe抗原含有免疫複合体の検出を試みた.Raji cell assayによるCICの陽性率は急性ウイルス肝炎(AVH) 34.2%(A型29.4%, B型41.7%,非A非B型33.7%),慢性肝炎(活動性)(CAH) 33.3%,慢性肝炎(非活動性)(CIH) 20.0%,肝硬変(LC) 28.1%,肝細胞癌(HCC) 33.3%,無症候性HBs抗原キャリア(ASC) 10.7...
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| Published in | 肝臓 Vol. 25; no. 2; pp. 168 - 177 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | Japanese |
| Published |
一般社団法人 日本肝臓学会
1984
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0451-4203 1881-3593 |
| DOI | 10.2957/kanzo.25.168 |
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| Abstract | Raji cell assayを用いて各種肝疾患における血中免疫複合体(CIC)の出現頻度,疾患活動性との関連について検討し,さらにB型肝疾患についてはPEG-trypsinisation assayによりHBs抗原,HBe抗原含有免疫複合体の検出を試みた.Raji cell assayによるCICの陽性率は急性ウイルス肝炎(AVH) 34.2%(A型29.4%, B型41.7%,非A非B型33.7%),慢性肝炎(活動性)(CAH) 33.3%,慢性肝炎(非活動性)(CIH) 20.0%,肝硬変(LC) 28.1%,肝細胞癌(HCC) 33.3%,無症候性HBs抗原キャリア(ASC) 10.7%,アルコール性肝障害30.0%,原発性胆汁性肝硬変(PBC) 60.0%でPBCで最も高率であった.各種B型肝疾患では2法によるCICの検出率は高い一致率を示し,CIC陽性例ではHBs抗原が高頻度に含有されていることが明らかになった.HBe抗原含有免疫複合体はAVH, CAH各1例に一過性に検出されたにすぎないが,HBs抗原含有免疫複合体は血中HBe抗原陽性例で高頻度に出現したことから疾患活動性との関連が示唆された. |
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| AbstractList | Raji cell assayを用いて各種肝疾患における血中免疫複合体(CIC)の出現頻度,疾患活動性との関連について検討し,さらにB型肝疾患についてはPEG-trypsinisation assayによりHBs抗原,HBe抗原含有免疫複合体の検出を試みた.Raji cell assayによるCICの陽性率は急性ウイルス肝炎(AVH) 34.2%(A型29.4%, B型41.7%,非A非B型33.7%),慢性肝炎(活動性)(CAH) 33.3%,慢性肝炎(非活動性)(CIH) 20.0%,肝硬変(LC) 28.1%,肝細胞癌(HCC) 33.3%,無症候性HBs抗原キャリア(ASC) 10.7%,アルコール性肝障害30.0%,原発性胆汁性肝硬変(PBC) 60.0%でPBCで最も高率であった.各種B型肝疾患では2法によるCICの検出率は高い一致率を示し,CIC陽性例ではHBs抗原が高頻度に含有されていることが明らかになった.HBe抗原含有免疫複合体はAVH, CAH各1例に一過性に検出されたにすぎないが,HBs抗原含有免疫複合体は血中HBe抗原陽性例で高頻度に出現したことから疾患活動性との関連が示唆された. |
| Author | 佐々木, 博 清水, 幸裕 紺田, 健彦 井上, 恭一 中野, 護 樋口, 清博 |
| Author_xml | – sequence: 1 fullname: 樋口, 清博 organization: 富山医科薬科大学第三内科 – sequence: 1 fullname: 紺田, 健彦 organization: 富山医科薬科大学第三内科 – sequence: 1 fullname: 中野, 護 organization: 富山医科薬科大学第三内科 – sequence: 1 fullname: 佐々木, 博 organization: 富山医科薬科大学第三内科 – sequence: 1 fullname: 井上, 恭一 organization: 富山医科薬科大学第三内科 – sequence: 1 fullname: 清水, 幸裕 organization: 富山医科薬科大学第三内科 |
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| References | 9) Hotta T, Kuriki J, Kakumu S: Loss of suppressor T cell function and circulating immune complexes in chronic active liver diseases. Clin Exp Immunol 47: 375-381, 1981 18) Goldberg MJ, Kaplan MM, Mitamura T: Evidence against an immune complex pathogenesis of primary biliary cirrhosis. Gastroenterology 83: 677-683, 1982 19) Takekoshi Y, Tanaka M, Miyakawa Y: Free small and IgG-associated large hepatitis B e antigen in the serum and glomerular capillary walls of two patients with membranous glomerulonephritis. N Eng J Med 300: 814-819, 1979 20) Takahashi K, Miyakawa Y, Gotanda T: Shift from free small hepatitis B e antigen to IgG-bound large form in tne circulating of human beings and chimpanzee acutely infected with hepatitis B virus. Gastroenterology 77: 1193-1199, 1979 15) Thomas HC, Potter BJ, Sherlock S: Is primary biliary cirrhosis an immune complex disease? Lancet ii: 1261-1263, 1977 17) Penner E, Albini B, Weiser M: Circulating and hepatic immune complexes in primary biliary cirrhosis. Gut 20: A908, 1979 2) Santro F, Watter P, Dessaint JP: Hepatitis B circulating immune complexes. Characterization by radioimmunoprecipitation-PEG assay (Ripega). J Immunol Methods 15: 201-209, 1977 14) Ohri H, Nagatuka Y, Yamada E: Characterization of immune complexes in sera from patients with non-A, non-B hepatitis. In: Viral hepatitis, Edited by W Szmuness, HJ Alter, JE Maynard, The Franklin Institute Press, Philadelphia, 1982, p331-338 4) Anh-Tuan N, Novak E: Detection and quantitation of hepatitis-B surface antigenspecific method. I. Detection and quantitation of in vitro prepared HBsAg-ICs. J Immunol Methods 33: 293-300, 1980 12) Thomas HC, Villiers DDE, Potter B: Immune complexes in acute and chronic liver disease. Clin Exp Immunol 31: 150-157, 1978 16) Gupta RC, Dickson ER, McDuffie FC: Circulating IgG complexes in primary biliary cirrhosis. A serial study in forty patients followed for two years. Clin Exp Immunol 34: 19-27, 1978 5) Anh-Tuan N, Novak E: Detection and quantitationn of hepatitis-B surface antigen immune complexes (HBsAg-Ics) by an antigenspecific method. II. Circulating immune complexes (CICs) in patients with hepatitis B and asymptomatic HBsAg carriers. J Immunol Methods 35: 307-318, 1980 3) Pemice W, Sodmann CP, Luben C: Antigen-specific detection of HBsAg-containing immune complexes in the course of hepatitis B virus infection. Clin Exp Immunol 37: 376-380, 1979 6) Gupta RC, Tan EM: Isolation of circulating immune complexes by conglutinin and separation of antigen from dissociated complexes by immobilized protein A. Clin Exp Immunol 46: 9-19, 1981 11) Creighton WD, Lambert PH, Miescher PA: Detection of antibodies annd soluble antigenantibody complexes by precipitation with polyethylene glycol. J Immunol 111: 1219-1227, 1973 1) Agnello V: Immune complex assays: The first ten years. Ann Intern Med 94: 266-267, 1981 8) 矢田純一,沢文博:培養株Raji細胞を用いた免疫複合体の検出法.免疫実験操作法VI.日本免疫学会編,日本免疫学会,金沢,1977, p1753-1756 13) Nydegger UE, lambert PH, Gerber H: Circulating immune complexes in the serum in systemic lupus erythematosus and in carriers of Hepatitis B antigen. Quantitation by binding to radiolabeled Clq. J Clin Invest 54: 297-309, 1974 10) Theofilopoulos AN, Wilson CB, Dixon FJ: The Raji cell radioimmune assay for detecting immune complexes in human sera. J Clin Invest 67: 169-182, 1976 7) 福田善弘,佐野万瑳寿,井村裕夫,他:Clq binding testによる肝疾患患者血中免疫複合体の測定とその病因的意義,陽性例における免疫複合体形成にあずかるHBV関連抗原抗体の検索.肝臓 23: 490-496, 1982 |
| References_xml | – reference: 8) 矢田純一,沢文博:培養株Raji細胞を用いた免疫複合体の検出法.免疫実験操作法VI.日本免疫学会編,日本免疫学会,金沢,1977, p1753-1756 – reference: 10) Theofilopoulos AN, Wilson CB, Dixon FJ: The Raji cell radioimmune assay for detecting immune complexes in human sera. J Clin Invest 67: 169-182, 1976 – reference: 19) Takekoshi Y, Tanaka M, Miyakawa Y: Free small and IgG-associated large hepatitis B e antigen in the serum and glomerular capillary walls of two patients with membranous glomerulonephritis. N Eng J Med 300: 814-819, 1979 – reference: 14) Ohri H, Nagatuka Y, Yamada E: Characterization of immune complexes in sera from patients with non-A, non-B hepatitis. In: Viral hepatitis, Edited by W Szmuness, HJ Alter, JE Maynard, The Franklin Institute Press, Philadelphia, 1982, p331-338 – reference: 15) Thomas HC, Potter BJ, Sherlock S: Is primary biliary cirrhosis an immune complex disease? Lancet ii: 1261-1263, 1977 – reference: 5) Anh-Tuan N, Novak E: Detection and quantitationn of hepatitis-B surface antigen immune complexes (HBsAg-Ics) by an antigenspecific method. II. Circulating immune complexes (CICs) in patients with hepatitis B and asymptomatic HBsAg carriers. J Immunol Methods 35: 307-318, 1980 – reference: 9) Hotta T, Kuriki J, Kakumu S: Loss of suppressor T cell function and circulating immune complexes in chronic active liver diseases. Clin Exp Immunol 47: 375-381, 1981 – reference: 12) Thomas HC, Villiers DDE, Potter B: Immune complexes in acute and chronic liver disease. Clin Exp Immunol 31: 150-157, 1978 – reference: 1) Agnello V: Immune complex assays: The first ten years. Ann Intern Med 94: 266-267, 1981 – reference: 7) 福田善弘,佐野万瑳寿,井村裕夫,他:Clq binding testによる肝疾患患者血中免疫複合体の測定とその病因的意義,陽性例における免疫複合体形成にあずかるHBV関連抗原抗体の検索.肝臓 23: 490-496, 1982 – reference: 18) Goldberg MJ, Kaplan MM, Mitamura T: Evidence against an immune complex pathogenesis of primary biliary cirrhosis. Gastroenterology 83: 677-683, 1982 – reference: 4) Anh-Tuan N, Novak E: Detection and quantitation of hepatitis-B surface antigenspecific method. I. Detection and quantitation of in vitro prepared HBsAg-ICs. J Immunol Methods 33: 293-300, 1980 – reference: 2) Santro F, Watter P, Dessaint JP: Hepatitis B circulating immune complexes. Characterization by radioimmunoprecipitation-PEG assay (Ripega). J Immunol Methods 15: 201-209, 1977 – reference: 16) Gupta RC, Dickson ER, McDuffie FC: Circulating IgG complexes in primary biliary cirrhosis. A serial study in forty patients followed for two years. Clin Exp Immunol 34: 19-27, 1978 – reference: 6) Gupta RC, Tan EM: Isolation of circulating immune complexes by conglutinin and separation of antigen from dissociated complexes by immobilized protein A. Clin Exp Immunol 46: 9-19, 1981 – reference: 11) Creighton WD, Lambert PH, Miescher PA: Detection of antibodies annd soluble antigenantibody complexes by precipitation with polyethylene glycol. J Immunol 111: 1219-1227, 1973 – reference: 13) Nydegger UE, lambert PH, Gerber H: Circulating immune complexes in the serum in systemic lupus erythematosus and in carriers of Hepatitis B antigen. Quantitation by binding to radiolabeled Clq. J Clin Invest 54: 297-309, 1974 – reference: 17) Penner E, Albini B, Weiser M: Circulating and hepatic immune complexes in primary biliary cirrhosis. Gut 20: A908, 1979 – reference: 20) Takahashi K, Miyakawa Y, Gotanda T: Shift from free small hepatitis B e antigen to IgG-bound large form in tne circulating of human beings and chimpanzee acutely infected with hepatitis B virus. Gastroenterology 77: 1193-1199, 1979 – reference: 3) Pemice W, Sodmann CP, Luben C: Antigen-specific detection of HBsAg-containing immune complexes in the course of hepatitis B virus infection. Clin Exp Immunol 37: 376-380, 1979 |
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| Snippet | Raji cell assayを用いて各種肝疾患における血中免疫複合体(CIC)の出現頻度,疾患活動性との関連について検討し,さらにB型肝疾患についてはPEG-trypsinisation assayによりHBs抗原,HBe抗原含有免疫複合体の検出を試みた.Raji cell... |
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| Title | 各種肝疾患における血中免疫複合体の臨床的意義およびB型肝疾患におけるHBs抗原,HBe抗原含有免疫複合体の同定について |
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