Effect of active oxygen on guanidine synthesis from various guanidino compounds in vitro

• Published in: Nihon Jinzo Gakkai shi Vol. 29; no. 11; pp. 1355 - 1364
• Main Authors: SAKAMOTO, MASAKO, AOYAGI, KAZUMASA, NAGASE, SOHJI, NARITA, MITSUHARU, TOJO, SHIZUO
• Format: Journal Article
• Language: Japanese
• Published: Japan Japanese Society of Nephrology 01.11.1987
• Subjects: active oxygen; Animals; arginine; Arginine - metabolism; Glycine - analogs & derivatives; Glycine - metabolism; Guanidine; Guanidines - metabolism; guanidinoacetic acid; In Vitro Techniques; iron; Liver - cytology; Liver - metabolism; Oxygen - pharmacology; Rats
• ISSN: 0385-2385; 1884-0728
• DOI: 10.14842/jpnjnephrol1959.29.1355

Active oxygen plays an important role in tissue injury. We have reported that methylguanidine (MG), a uremic toxin, is formed by active oxygen from creatinine (Cr) in vitro and in isolated rat hepatocytes. In this study, we investigate the role of active oxygen in the synthesis of guanidine (G) of which serum level increased in uremics. Substances were incubated with active oxygen sources in 50 mM phosphate buffer (pH 7.4 at 37°C). We used xanthine oxidase system for the superoxide radical, hydrogen peroxide (H2O2) and H2O2 + FeC12 for the hydroxyl radical (OH) as active oxygen sources. G was formed from various guanidino compounds including arginine, guanidinoacetic acid (GAA), canavanine, creative, Cr and MG by OH. And G synthesis from arginine and GAA by every kind of active oxygen was inhibited by the addition of corresponding scavengers. Among active oxygen species OH produced the largest amount of G. These results indicate that G is formed from various guanidino compounds as a result of active oxygen.