ASSAY METHODS FOR CARUMONAM IN BIOLOGICAL SPECIMENS

• Published in: CHEMOTHERAPY Vol. 35; no. Supplement2; pp. 178 - 186
• Main Authors: FUGONO, TAKESHI, MAEDA, KENICHI
• Format: Journal Article
• Language: English; Japanese
• Published: Japanese Society of Chemotherapy 1987
• ISSN: 0009-3165; 1884-5894
• DOI: 10.11250/chemotherapy1953.35.Supplement2_178

Assay methods for pharmacokinetic studies of carumonam and its metabolites were developed. For microbiological determination of carumonam, an agar-well method, using Escherichia coli NIHJ as the test organism and antibiotic medium No.4 (DAIGO) as the test medium, was established. The sensitivity of the method for carumonam was about 0.1μg (potency)/ml. A method with higher sensitivity was also elaborated by using Providencia rettgeri ATCC 9250 and DST agar supplemented with 0.15% bile salts and 0.2mg% crystal violet; the sensitivity of this method was about 0.02μg (potency)/ml. A bioautographic method using the same Providencia strain, was devised for detection of antibacterially active metabolites. Carumonam and its open, β-lactam ring hydrolysis product, Related Compound I, was quantitatively determined by an HPLC method on a Nucleosil 5C18 column using 0.005M tetrabutylammonium hydrogen sulfate (pH 3.0)-acetonitrile (85:15v/v) as a mobile phase.