Effect of peptide derived from Emdogain^[○!R] on human periodontal ligament fibroblasts

• Published in: Journal of Osaka Dental University Vol. 43; no. 1; pp. 111 - 117
• Main Authors: Kawanaka, Ayako, Tominaga, Kazuya, Tanaka, Akio
• Format: Journal Article
• Language: English
• Published: Osaka Odontological Society 2009
• Subjects: Bone-related genes; Cell proliferation; Enamel matrix derivative; Gene expression; Periodontal ligament fibroblasts; Synthetic peptide
• ISSN: 0475-2058; 2189-6488
• DOI: 10.18905/jodu.43.1_111

There are several methods for regeneration of periodontal tissue damaged by periodontal disease. Although use of enamel matrix derivative (EMD) is one of the most effective, its mechanism is not well understood. The present study was designed to elucidate the mechanism of EMD. We explored how the proliferation, differentiation and gene expression of human periodontal ligament fibroblasts (HPdLF) are influenced by synthetic peptides derived from EMD. In addition, we carried out probe-target hybridization labeled by fluorophore to the expressed target genes and their microarray analysis. The amount of HPdLF cell DNA was increased and alkaline phosphatase activity was decreased during culture in the medium with synthetic peptides. A total number of 28,563 instances of gene expression were observed. Microarray analysis showed that although bone morphogenetic protein (BMP) receptor type 1 A, BMP 4, osteonectin, and BMP receptor type 1 B gene expression were increased, fibroblast growth factor receptor-like protein 1 gene was decreased. Thus, microarray analysis showed that the expression of genes related to osteogenesis was increased with the use of the peptides. The present results indicate that the peptide derived from EMD is effective in periodontal tissue regeneration.