Detection of Steps and Rotation in the Gliding Motility of Mycoplasma mobile

Mycoplasma mobile is one of the fastest gliding bacteria, gliding with a speed of 4.5 μm s . This gliding motility is driven by a concerted movement of 450 supramolecular motor units composed of three proteins, Gli123, Gli349, and Gli521, in the gliding motility machinery. With general experimental...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2646; p. 327
Main Authors Kinosita, Yoshiaki, Sugawa, Mitsuhiro, Miyata, Makoto, Nishizaka, Takayuki
Format Journal Article
LanguageEnglish
Published United States 2023
Subjects
Bacterial Proteins - metabolism
Movement
Mycoplasma - metabolism
Rotation
Stepwise movement
Motility
Sialyllactose
Optical microscope
Mycoplasma mobile
Bacteria
Rotation
Gliding
Single-molecular techniques
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Summary:Mycoplasma mobile is one of the fastest gliding bacteria, gliding with a speed of 4.5 μm s . This gliding motility is driven by a concerted movement of 450 supramolecular motor units composed of three proteins, Gli123, Gli349, and Gli521, in the gliding motility machinery. With general experimental setups, it is difficult to obtain the information on how each motor unit works. This chapter describes strategies to decrease the number of active motor units to extract stepwise cell movements driven by a minimum number of motor units. We also describe an unforeseen motility mode in which the leg motions convert the gliding motion into rotary motion, which enables us to characterize the motor torque and energy-conversion efficiency by adding some more assumptions.
ISSN:1940-6029
DOI:10.1007/978-1-0716-3060-0_27