Social Motility Assays of Flavobacterium johnsoniae

Flavobacterium johnsoniae cells move rapidly over solid surfaces by gliding motility. The collective migration of F. johnsoniae on the surfaces results in the formation of spreading colonies. Colony spreading is influenced by adhesin components on the cell surface and the concentrations of agar and...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 2646; p. 287
Main Authors Sato, Chikara, Sato, Keiko
Format Journal Article
LanguageEnglish
Published United States 2023
Subjects
Adhesins, Bacterial - metabolism
Agar
Bacterial Proteins - metabolism
Flavobacterium - metabolism
Locomotion
Gliding motility
Surface adhesin
Social motility
Colony spreading
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Summary:Flavobacterium johnsoniae cells move rapidly over solid surfaces by gliding motility. The collective migration of F. johnsoniae on the surfaces results in the formation of spreading colonies. Colony spreading is influenced by adhesin components on the cell surface and the concentrations of agar and glucose. For example, on nutrient-poor agar media, film-like, round spreading colonies are formed. F. johnsoniae displays at least two types of migration: small cell cluster movements leading to concentric colonies and individual cell movements leading to dendritic colonies. The methods for observing colony morphology are described in this chapter.
ISSN:1940-6029
DOI:10.1007/978-1-0716-3060-0_23