高ヒスチジン糖タンパク質は好中球恒常性維持に寄与する多面的機能を有する

• Published in: 日本薬理学会年会要旨集 p. 1-B-P-003
• Main Authors: ハティポール, オメル ファルク, 髙橋, 英夫, 森, 秀治, 西堀, 正洋, 和氣, 秀徳, 西中, 崇
• Format: Journal Article
• Language: Japanese
• Published: 公益社団法人 日本薬理学会 2023
• Subjects: coagulo-fibrinolytic system; immune system; neutrophil; phagocytosis
• ISSN: 2435-4953
• DOI: 10.1254/jpssuppl.97.0_1-B-P-003

Histidine-rich glycoprotein (HRG) is a plasma glycoprotein present in blood at a concentration of approximately 1 µM. It is known to have various physiological activities related to the homeostasis of the coagulation-fibrinolytic system, the immune system, and the blood vascular system, and until now, its effects on monocytes, lymphocytes, and platelets have been reported, but its effects on neutrophils have not been reported. Therefore, we investigated the effects of HRG on neutrophils. In the presence of HRG, neutrophils maintain a regular spherical morphology and smooth cell surface without microvilli, and F-actin polymerizes just beneath the cell membrane, giving them moderate elasticity. However, in an environment with low or no levels of HRG, such as sepsis, we found that neutrophils show reduced cellular elasticity, an amoeba-like morphology, increased cell surface microvilli, and activation of adhesion molecules such as CD 11 b, CD 62 L, and CD 162, leading to increased adhesion and frictional resistance to blood vessels. Decreased HRG also affects neutrophil functions, resulting in increased the release of NETs and ROS. In addition, the reduction markedly decreased their migration to bacteria and phagocytosis. These results indicate that HRG is a critical important protein for maintaining neutrophil homeostasis.