Hemokinin-1誘発性疼痛関連行動におけるTRPV1チャネルの関与

• Published in: 日本薬理学会年会要旨集 p. 2-P-128
• Main Authors: 林, 貴史, 菅野, 秀一, 渡辺, 千寿子, 我妻, 恭行, 原, 明義, 櫻田, 司, 櫻田, 忍
• Format: Journal Article
• Language: Japanese
• Published: 公益社団法人 日本薬理学会 2022
• Subjects: neuropeptide; nociception; spinal cord; tachykinin
• ISSN: 2435-4953
• DOI: 10.1254/jpssuppl.95.0_2-P-128

Hemokinin-1 mainly acts on the neurokinin-1 (NK1) receptors and is involved in the pain transmission like substance P. Transient receptor potential vanilloid 1 (TRPV1) channel are activated by various factors, but it has not been reported that TRPV1 channel is activated by hemokinin-1. We found that the nociceptive behaviors by intrathecally (i.t.) administered hemokinin-1 in mice were caused through TRPV1 channel.The nociceptive behaviors induced by i.t. administered hemokinin-1 were observed directed biting and licking along with both hindlimb scratching in mice and recorded as the total response times of these nociceptive behaviors. In order to confirm that Hemokinin-1 activated TRPV1, the amount of phosphorylated TRPV1 was measured by Western blot analysis.The nociceptive behaviors induced by i.t. administered hemokinin-1 was decreased by capsazepine, a TRPV1 channel antagonist. Pretreatment with antiserum against TRPV1 channel was eliminated the nociceptive behaviors induced by i.t. administered hemokinin-1. The phosphorylation of TRPV1 channel in the spinal dorsal horn was increased by i.t. administered hemokinin-1. These results show that hemokinin-1, unlike substance P, elicits the nociceptive behaviors by TRPV1 channel as well as NK1 receptor on spinal dorsal horn.