てんかん原性初期のマウス海馬におけるFosl1遺伝子発現

• Published in: 日本薬理学会年会要旨集 p. 3-P-199
• Main Authors: 伊藤, 康一, 小森, 理絵, 石原, 康宏, 松尾, 平
• Format: Journal Article
• Language: Japanese
• Published: 公益社団法人 日本薬理学会 2022
• Subjects: gene expression; hippocampus; microglia
• ISSN: 2435-4953
• DOI: 10.1254/jpssuppl.95.0_3-P-199

We recently reported that LPS markedly increased Fosl1 (Fra1, one of Fos family) expression, and knockdown of Fosl1 significantly suppressed the expression of inflammatory cytokines in murine BV-2 microglial cell lines. These observations suggest that Fosl1 is involved in microglial inflammatory activation. Our previous studies also showed that inflammation in the hippocampus at the early phase of the epileptogenic processes could induce spontaneous seizures in pilocarpine (PILO)-induced status epilepticus (SE) mouse model. Therefore, in this study we clarified relationship between Fosl1 expression and inflammation in the hippocampus after PILO-SE. Real-time PCR analyses showed that expression of hippocampal Fosl1 displayed more than a 100-fold increase on the 1st and 2nd days after PILO-SE, then gradually decreased and still high level (45-fold of control) on the 7th day. Next, in order to identify the cell types expressing Fosl1, neurons, glial cells (astroglia and microglia), and vascular endothelial cells were separated from hippocampus of PILO-SE and control mouse using a FACSAria II (BD). The expression analyses showed that hippocampal Fosl1 was mainly expressed in the glial cells. The relationship between increased hippocampal Fosl1 expression after PILO-SE and intrahippocampal inflammation is under investigation.