Comparison of the Closed Epicutaneous Test and Adjuvant Patch Test Using Four Tar-Colored Substances

• Published in: Skin research Vol. 29; no. 3; pp. 542 - 547
• Main Authors: KINOSHITA, Miwako, ISHIHARA, Masaru, ITOH, Masatoshi, NISHIMURA, Makoto, YAMADA, Koji, NOIKE, Naomi
• Format: Journal Article
• Language: English; Japanese
• Published: Meeting of Osaka Dermatological Association 1987
• Subjects: adjuvant patch test (APT); closed epicutaneous test (CET); guinea pig maximization test (GMT); tar-colored substances
• ISSN: 0018-1390; 1884-541X
• DOI: 10.11340/skinresearch1959.29.542

Skin irritability and sensitization potency of4different tar-colored substances (red No.207, red No.223, blue No.205, and yellow No.204) were studied on female guinea pigs. Both skinirritation tests and sensitization testswere performed. Skin irritation tests: The four colored substanceswere tested in concentrations rangingfrom 0. 01% to 30%. No positive reactionswere observed at any concentration. Sensitization tests: The closed epicutaneoustest (CET) and the adjuvant patch test (APT) were performed. When a 30% concentration of red No.223was applied, neither CET nor APT showed anysensetized animals. With a 30% concentrationof blue No.205, APT showed 70% of theanimals to be sensitized. Using 30% red No.207, APT results showed 90%, and CETshowed 50% of the animals to be sensitized.When 30% yellow No.204 was used, APTfound 100% of the animals to be sensitized, while CET found 70%. These results seem to indicate that APT isa more sensitive sensitization test than CET.